Dark-induced accumulation of a basic pathogenesis-related (PR-1) transcript and a light requirement for its induction by ethylene

• Published in: Plant molecular biology Vol. 19; no. 4; pp. 589 - 599
• Main Authors: Eyal, Y. (Weizmann Inst. of Science, Rehovot (Israel). Dept. of Plant Genetics), Sagee, O, Fluhr, R
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer 01-07-1992
• Subjects: Amino Acid Sequence; Base Sequence; Biological and medical sciences; Blotting, Northern; Cloning, Molecular; CODE GENETIQUE; CODIGO GENETICO; Darkness; DNA; ETEFON; ETHEPHON; ETHYLENE; Ethylenes - pharmacology; ETILENO; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation - drug effects; Gene Expression Regulation - radiation effects; genes; GENETIC CODE; induction; Light; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; NICOTIANA TABACUM; nucleotide sequence; PATHOGENESE; PATHOGENESIS; pathogenesis-related proteins; PATOGENESIS; Plant Proteins - genetics; Plant Proteins - metabolism; predictions; PROTEINAS; PROTEINE; PROTEINS; Sequence Alignment; Signal Transduction; Transcription, Genetic; Transcription. Transcription factor. Splicing. Rna processing; Light effect; Nucleotide sequence; Induction; Acidic protein; Sequence alignment; Ethylene; Homology; Stimulant plant; Gene expression; Nicotiana tabacum; Messenger RNA; Basic protein; Dicotyledones; Angiospermae; Spermatophyta; Solanaceae; Comparative study; Aminoacid sequence
• ISSN: 0167-4412; 1573-5028
• DOI: 10.1007/bf00026785

A gene encoding a basic-type pathogenesis-related protein from Nicotiana tabacum (prb-1b) was cloned, sequenced and characterized. It contains an open reading frame of 179 amino acids that is ca. 65% homologous with the acidic PR-1 class of pathogenesis-related proteins and 87% homologous with a different basic-type PR-1 gene. In the light, physiological levels of ethylene rapidly (1 h) induced basic, but not acidic-type, PR-1 transcript. Additional elicitors acting via ethylene, such as alpha-aminobutyric acid, were shown to induce basic- and acidic-type PR-1 transcript accumulation in a light-dependent manner. In contrast, xylanase, an ethylene-independent elicitor, induced transcript accumulation of basic- and acidic-type PR-1 in a light-independent manner. Dark-induced accumulation of basic PR-1 transcript occurred at night in greenhouse-grown plants and, to a greater extent, in continuously dark-treated plants. The novel dark regulation may point to additional nonpathogenesis-related roles for these genes in plant-environment interactions.