Increase in synaptic hippocampal zinc concentration following chronic but not acute zinc treatment in rats

• Published in: Brain research Vol. 1090; no. 1; pp. 69 - 75
• Main Authors: Szewczyk, Bernadeta, Sowa, Magdalena, Czupryn, Artur, Wierońska, Joanna M., Brański, Piotr, Sadlik, Krystyna, Opoka, Włodzimierz, Piekoszewski, Wojciech, Śmiałowska, Maria, Skangiel-Kramska, Jolanta, Pilc, Andrzej, Nowak, Gabriel
• Format: Journal Article
• Language: English
• Published: London Elsevier B.V 23-05-2006; Amsterdam Elsevier; New York, NY
• Subjects: Animals; Biochemistry and metabolism; Biological and medical sciences; Central nervous system; Dose-Response Relationship, Drug; Drug Administration Schedule; Electroshock; Fundamental and applied biological sciences. Psychology; Growth Cones - metabolism; Growth Cones - ultrastructure; Hippocampus; Hippocampus - anatomy & histology; Hippocampus - drug effects; Hippocampus - metabolism; Injections, Intraperitoneal; Male; Neuronal Plasticity - physiology; Predictive Value of Tests; Presynaptic Terminals - drug effects; Presynaptic Terminals - metabolism; Rat; Rats; Rats, Wistar; Spectrophotometry, Atomic; Synaptic Transmission - drug effects; Synaptic Transmission - physiology; Synaptic zinc; Up-Regulation - drug effects; Up-Regulation - physiology; Vertebrates: nervous system and sense organs; Zinc - analysis; Zinc - metabolism; Zinc - pharmacology; Zinc Compounds - analysis; Zinc Compounds - metabolism; Zinc Compounds - pharmacology; Zinc treatment; Synaptic zinc; Zinc treatment; Rat; Hippocampus; Acute; Rodentia; Central nervous system; Zinc; Encephalon; Vertebrata; Chronic; Synapse; Mammalia; Treatment; Animal
• ISSN: 0006-8993; 1872-6240
• DOI: 10.1016/j.brainres.2006.03.035

Electroconvulsive seizures (ECS), one of the most effective treatments of depression, induce mossy fiber sprouting (when assayed by means of synaptic zinc method), and this indicates an increase in the synaptic zinc level in the hippocampus following such therapy. The aim of the present study was to investigate the influence of acute and chronic zinc hydroaspartate administration on the synaptic and total zinc level in the rat hippocampus. We used two methods of zinc determination: (1) zinc-selenium method, which images the pool of synaptic zinc, and (2) flame atomic absorption spectrometry, which assays the total concentration of zinc. Our results indicate that chronic (14 × 65 mg/kg), but not acute, zinc hydroaspartate administration intraperitoneally (i.p.) increases the pool of synaptic zinc in the majority of rat hippocampal layers (by 72–190%), except for the stratum moleculare and stratum radiatum CA, and perforant path DG. On the other hand, no changes were found in total hippocampal zinc level, measured by flame atomic absorption spectrometry. These data suggest that chronic zinc treatment increases the pool of synaptic zinc in the hippocampus, and this effect is similar to that observed following chronic ECS treatment. The measurement of zinc concentration in the whole hippocampus by the flame atomic absorption spectrometry method is not sensitive enough to detect such subtle alteration.