Gas-chromatographic determination of trace amounts of tetrodotoxin in water, drugs, and blood plasma

• Published in: Journal of analytical chemistry (New York, N.Y.) Vol. 71; no. 3; pp. 289 - 296
• Main Authors: Stan’kov, I. N., Kondrat’ev, V. B., Derevyagina, I. D., Morozova, O. T., Selivanova, V. I., Semchenko, F. M., Rufanov, K. A., Dvoretskaya, S. I.
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 01-03-2016; Springer
• Subjects: Analytical Chemistry; Blood plasma; Chemical properties; Chemistry; Chemistry and Materials Science; Composition; Drugs; Gas chromatography; Identification and classification; Methods; Tetrodotoxin; Trace analysis; Water; mass spectrometry; biological medium; tetrodotoxin; blood plasma; gas–liquid chromatography
• ISSN: 1061-9348; 1608-3199
• DOI: 10.1134/S1061934816030138

A method has been developed for the determination of tetrodotoxin in water, blood plasma, and drugs at a level of 0.05—1.0 µg/mL. The method is based on the hydrolytic decomposition of tetrodotoxin by a sodium hydroxide solution, extraction of 2-amino-6-hydroxymethyl-8-hydroxyquinazoline using liquid–liquid extraction, preparation of its derivative by reaction with
N,O
-bis(trimethylsilyl)trifluroacetamide, and the determination of the derivative by gas–liquid chromatography with a mass-spectrometric detector.