Reversed-phase high-pressure liquid chromatographic analysis of sulconazole in plasma
A sensitive and specific analytical method for the measurement of sulconazole in plasma is described. The compound was extracted from plasma at pH 10 with hexane-methylene chloride. Samples were subjected to high-pressure liquid chromatography (HPLC) using an acetonitrile-phosphate buffer mixture as...
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| Published in: | Journal of pharmaceutical sciences Vol. 70; no. 12; p. 1338 |
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| Main Authors: | , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
United States
01-12-1981
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| Subjects: | |
| Online Access: | Get more information |
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| Summary: | A sensitive and specific analytical method for the measurement of sulconazole in plasma is described. The compound was extracted from plasma at pH 10 with hexane-methylene chloride. Samples were subjected to high-pressure liquid chromatography (HPLC) using an acetonitrile-phosphate buffer mixture as the mobile phase. The components of interest were measured using a variable-wavelength detector at 229 nm. Sulconazole concentrations of greater than or equal to 0.5 microgram/ml can be measured with confidence using this method. Linear calibration curves were constructed over the concentration range of 0.5-5 microgram/ml for sulconazole from dog plasma. A dog was administered a single oral 1000-mg dose of tritiated sulconazole nitrate; total plasma radioactivity and sulconazole plasma levels determined by HPLC are reported. |
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| ISSN: | 0022-3549 |
| DOI: | 10.1002/jps.2600701212 |