Intra-Specific Latitudinal Clines in Leaf Carbon, Nitrogen, and Phosphorus and their Underlying Abiotic Correlates in Ruellia Nudiflora

• Published in: Scientific reports Vol. 8; no. 1; pp. 596 - 8
• Main Authors: Abdala-Roberts, Luis, Covelo, Felisa, Parra-Tabla, Víctor, Terán, Jorge C. Berny Mier y, Mooney, Kailen A., Moreira, Xoaquín
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 12-01-2018; Nature Publishing Group
• Subjects: 631/158/2450; 631/158/857; 64; Abiotic factors; Acanthaceae - chemistry; Carbon; Carbon - analysis; Cation exchange; Clines; Humanities and Social Sciences; Leaves; multidisciplinary; Nitrogen; Nitrogen - analysis; Nutrients; Phenotype; Phosphorus; Phosphorus - analysis; Plant Leaves - chemistry; Precipitation; Rain; Science; Science (multidisciplinary); Seasons; Stoichiometry; Stress, Physiological; Temperature; Variation
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-017-18875-w

While plant intra-specific variation in the stoichiometry of nutrients and carbon is well documented, clines for such traits have been less studied, despite their potential to reveal the mechanisms underlying such variation. Here we analyze latitudinal variation in the concentration of leaf nitrogen (N), phosphorus (P), carbon (C) and their ratios across 30 populations of the perennial herb
Ruellia nudiflora
. In addition, we further determined whether climatic and soil variables underlie any such latitudinal clines in leaf traits. The sampled transect spanned 5° latitude (ca. 900 km) and exhibited a four-fold precipitation gradient and 2 °C variation in mean annual temperature. We found that leaf P concentration increased with precipitation towards lower latitudes, whereas N and C did not exhibit latitudinal clines. In addition, N:P and C:P decreased towards lower latitudes and latitudinal variation in the former was weakly associated with soil conditions (clay content and cation exchange capacity); C:N did not exhibit a latitudinal gradient. Overall, these results emphasize the importance of addressing and disentangling the simultaneous effects of abiotic factors associated with intra-specific clines in plant stoichiometric traits, and highlight the previously underappreciated influence of abiotic factors on plant nutrients operating under sharp abiotic gradients over smaller spatial scales.