Baculovirus vectors for efficient gene delivery and expression in mammalian cells

• Published in: Molecular biology (New York) Vol. 44; no. 3; pp. 479 - 487
• Main Authors: Koroleva, N. N, Spirin, P. V, Timokhova, A. V, Rubtzov, P. M, Kochetkov, S. N, Prasolov, V. S, Beljelarskaya, S. N
• Format: Journal Article
• Language: English
• Published: Dordrecht Dordrecht : SP MAIK Nauka/Interperiodica 01-06-2010; SP MAIK Nauka/Interperiodica; Springer Nature B.V
• Subjects: Animals; Baculoviridae; Baculovirus; Biochemistry; Biomedical and Life Sciences; Cell Line, Tumor; Cells; Cercopithecus aethiops; COS Cells; Cytomegalovirus; Gene Expression; Hepacivirus - genetics; Hepatitis C virus; Human Genetics; Humans; Life Sciences; Mammals; Other Problems; Recombinant Proteins - biosynthesis; Recombinant Proteins - genetics; Spodoptera; Transduction, Genetic; Viral Structural Proteins - biosynthesis; Viral Structural Proteins - genetics; hepatitis C virus (HCV); HCV-like particles; COS-7 and Huh7; baculovirus AcMNPV; BacMam virus; HCV structural proteins; mammalian cells HEK293T; Sf9 insect cells
• ISSN: 0026-8933; 0026-8984; 1608-3245
• DOI: 10.1134/S0026893310030180

Baculovirus expression vectors are extensively used for the delivery of foreign genes and expression of recombinant proteins in insect and mammalian cells. Modified baculoviruses containing mammalian promoter elements (BacMam viruses) for an efficient transient and stable transduction of diverse mammalian cells ensure a high level of heterologous protein expression both in vitro and in vivo. Recombinant baculovirus vectors containing mammalian expression cassette with cytomegalovirus promoter, green or red fluorescent protein gene, SV40pA polyadenylation signal, and polylinker MCS were constructed for the delivery of genes encoding hepatitis C virus structural proteins into mammalian cells. In HEK293T and Huh7 cells, formation of glycoprotein complexes and HCV4ike particles was observed. A high efficiency of the baculovirus-medi-ated gene transfer and expression of the virus envelope proteins in mammalian cells was demonstrated using fluorescence, flow cytometry, and immunoblot techniques.