Genome-derived cytosolic DNA contributes to type I interferon expression and immunogenicity of B-cell lymphoma cells

• Published in: Cytokine (Philadelphia, Pa.) Vol. 76; no. 2; pp. 581 - 582
• Main Authors: Shen, Yu J., Ho, Samantha S.W., Tan, Nikki Y., Koo, Christine Xing’Er, Khatoo, Muznah, Cheung, Florence S.G., Gasser, Stephan
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01-12-2015
• Subjects: Cancer; Cell Line, Tumor; DNA - genetics; DNA sensors; Genome; Humans; Innate immunity; Interferon Type I - genetics; Lymphoma, B-Cell - genetics; Lymphoma, B-Cell - immunology; Non-B DNA motifs; RNA - genetics; Type I interferon; Innate immunity; Type I interferon; Non-B DNA motifs; Cancer; DNA sensors
• ISSN: 1043-4666; 1096-0023
• DOI: 10.1016/j.cyto.2015.05.024

•Genome-derived ssDNA, dsDNA and DNA:RNA hybrids are present in the cytosol of tumor cells.•Cytosolic DNA can potentially form non-B DNA structures.•Cytosolic DNA contributes to type I interferon-dependent immune rejection of B-cell lymphoma.
We recently provided evidence that genome-derived DNA is present in the cytosol of many tumor cells. Genomic loci that give rise to cytosolic DNA can potentially form non-B DNA structures including triple-stranded RNA:DNA structures (R-loops). The RNA:DNA-specific endonuclease RNaseh1 reduced the levels of cytosolic DNA and type I interferon-dependent rejection of B-cell lymphoma suggesting that cytosolic DNA may contribute to immune surveillance of B-cell lymphoma.