Antimicrobial activity of diterpenoids from hairy roots of Salvia sclarea L.: Salvipisone as a potential anti-biofilm agent active against antibiotic resistant Staphylococci

• Published in: Phytomedicine (Stuttgart) Vol. 14; no. 1; pp. 31 - 35
• Main Authors: Kuźma, Ł., Różalski, M., Walencka, E., Różalska, B., Wysokińska, H.
• Format: Journal Article
• Language: English
• Published: Germany Elsevier GmbH 01-01-2007
• Subjects: Anti-Bacterial Agents - administration & dosage; Anti-Bacterial Agents - pharmacology; Anti-Bacterial Agents - therapeutic use; Antimicrobial activity; Diterpenes - administration & dosage; Diterpenes - pharmacology; Diterpenes - therapeutic use; Diterpenoids; Gram-Negative Bacteria - drug effects; Gram-Positive Bacteria - drug effects; Humans; Methicillin Resistance; Microbial Sensitivity Tests; Phytotherapy; Plant Extracts - administration & dosage; Plant Extracts - pharmacology; Plant Extracts - therapeutic use; Plant Roots; Resistant Staphylococcus spec; Salvia; Salvia sclarea L; Salvipisone; Staphylococcus aureus - drug effects; Diterpenoids; Antimicrobial activity; Salvia sclarea L; Salvipisone; Resistant Staphylococcus spec
• ISSN: 0944-7113; 1618-095X
• DOI: 10.1016/j.phymed.2005.10.008

The antimicrobial activities of crude dichloromethane fractions from acetone extracts of
Agrobacterium rhizogenes transformed roots and roots of field-grown plants of
Salvia sclarea as well as four pure abietane diterpenoids isolated from the hairy root cultures were determined. The growth of Gram-positive bacteria (
Staphylococcus aureus,
S. epidermidis,
Enterococcus faecalis) but not Gram-negative ones (
Escherichia coli,
Pseudomonas aeruginosa) or pathogenic fungi (
Candida albicans) was inhibited by fractions tested at concentrations of 37.5–75.0
μg
ml
−1. Abietane diterpenoids: salvipisone, aethiopinone, 1-oxoaethiopinone and ferruginol were shown to be bacteriostatic as well as bacteriocidal for the cultures of
S. aureus and
S. epidermidis strains, regardless of their antibiotic susceptibility profile. This was demonstrated by using simultaneously the optical density measuring method and 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide-reduction assay. The highest activity was shown by salvipisone which demonstrated also a very interesting activity when its effect on 24-h-old staphylococcal biofilm cells viability was examined. It limited the survival of biofilms formed by
S. aureus as well as by
S. epidermidis, putting this compound to the list of potential anti-biofilm agents, better than most of known antibiotics.