Fluorometric immunoassay for detecting the plant virus Citrus tristeza using carbon nanoparticles acting as quenchers and antibodies labeled with CdTe quantum dots

• Published in: Mikrochimica acta (1966) Vol. 183; no. 7; pp. 2277 - 2287
• Main Authors: Shojaei, Taha R., Salleh, Mohamad A. Mohd, Sijam, Kamaruzaman, Rahim, Raha A., Mohsenifar, Afshin, Safarnejad, Reza, Tabatabaei, Meisam
• Format: Journal Article
• Language: English
• Published: Vienna Springer Vienna 01-07-2016; Springer; Springer Nature B.V
• Subjects: Analytical Chemistry; Antibodies; Cadmium tellurides; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Citrus; Citrus fruits; Enzyme-linked immunosorbent assay; Enzymes; Fluorescence; Immunoassay; Intermetallic compounds; Linearity; Microengineering; Nanochemistry; Nanoparticles; Nanotechnology; Original Paper; Quantum dots; Quenching; Tetracycline; Tetracyclines; Viral antibodies; Viruses; Stern-Volmer relationship; Bioassay; Synthesis; FTIR; Bioconjugation; Competitive quenching; TEM; CdTe; ELISA
• ISSN: 0026-3672; 1436-5073
• DOI: 10.1007/s00604-016-1867-7

Cadmium-telluride quantum dots (QDs) were conjugated to an antibody (Ab) against Citrus tristeza virus (CTV), while the coat protein (CP) of the CTV was immobilized on the surface of carbon nanoparticles (CNPs). Following immunobinding of the QD-Ab and the CP-loaded CNPs, the fluorescence of the CdTe QDs was quenched by the CNPs. This effect was exploited to design a detection assay for the CTV which was found more sensitive and specific than the existing enzyme linked immunosorbent assay (ELISA). The limit of detection was measured at about 220 ng⋅ mL‾
1
of CTV. The Stern-Volmer plot of the CNPs-QD quencher pair showed a positive deviation from linearity which was ascribed to the presence of both static and dynamic quenching.
Graphical abstract
Cadmium-telluride quantum dots (QDs) were conjugated to an antibody (Ab) against Citrus tristeza virus (CTV), while the coat protein (CP) of the CTV was immobilized on the surface of carbon nanoparticles (CNPs). Following immunobinding of the QD-Ab and the CP-loaded CNPs, the fluorescence of the CdTe QDs was quenched by the CNPs. This effect was exploited to design a detection assay for the CTV which was found more sensitive and specific than the existing enzyme linked immunosorbent assay (ELISA).