Evaluation of longitudinal passive immunity transfer against lumpy skin disease virus in calves by different serological methods

• Published in: Veterinary research communications Vol. 48; no. 4; pp. 2797 - 2804
• Main Authors: Samojlović, Milena, Petrović, Tamaš, Polaček, Vladimir, Lupulović, Diana, Lazić, Gospava, Rogan, Dragan, Lazić, Sava
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01-08-2024; Springer Nature B.V
• Subjects: Animals; Antibodies; Antibodies, Viral - blood; Biomedical and Life Sciences; Brief Report; Cattle; Colostrum; Enzyme-linked immunosorbent assay; Enzyme-Linked Immunosorbent Assay - veterinary; Farms; Female; Immune response; Immunity (Disease); Immunity (passive); Immunity, Maternally-Acquired - immunology; Life Sciences; Longitudinal Studies; Lumpy skin disease; Lumpy Skin Disease - immunology; Lumpy Skin Disease - prevention & control; Lumpy Skin Disease - virology; Lumpy skin disease virus - immunology; Neutralization Tests - veterinary; Serbia; Serological tests; Serology; Skin diseases; Vaccination - veterinary; Veterinary Medicine/Veterinary Science; Viral Vaccines - immunology; Viruses; Zoology; Serbia; Antibodies; Passive immunity; Lumpy skin disease; VNT; Vaccination; ELISA
• ISSN: 0165-7380; 1573-7446; 1573-7446
• DOI: 10.1007/s11259-024-10421-0

To implement effective lumpy skin disease (LSD) control measures, such as timely vaccination, particularly in calves and serological monitoring, it is necessary to evaluate immune response after vaccination, both in adult cattle and in their calves. The aim of this study was to evaluate passive immunity transfer and duration of maternal antibodies against lumpy skin disease virus (LSDV) in calves born to vaccinated cows by two different serological methods. The longitudinal study was carried out on two farms in Serbia where no cases were reported during LSD outbreak in 2016. Fifteen cows on each farm were vaccinated and revaccinated with attenuated vaccine - Neethling strain. A total of 30 cows and 30 calves on both farms were included in the study. Serum samples from cows were collected on calving day and serum samples from their respective calves on days 10, 20, 30, 45, 60, 75, 90, 105 and 120 after birth. Colostrum samples were collected only from 15 cows on one farm. In order to determine the presence of antibodies against LSDV a total of 30 cow sera samples, 15 colostrum samples and 270 calf sera samples were examined by commercial enzyme-linked immunosorbent assay (ELISA) and modified virus neutralization test (VNT). Overall, the performance of both serological tests was very satisfactory. The results of this longitudinal study showed that persistence of passive immunity in calves is less than 4 months, and that most calves are not protected against LSDV at that age. Since the vaccination is the most important control measure against LSDV, the recommended age of six months for vaccination of calves born to vaccinated cows should be reassessed to achieve the most optimal protection against LSD.