Isolation of a lipase-producing Trichosporon spp and enzyme extraction by two-phase aqueous system

• Published in: Brazilian journal of microbiology Vol. 38; no. 1; pp. 62 - 64
• Main Authors: Santos, Juliana A.(University of Pernambuco Institute of Biological Sciences ,Federal University of Pernambuco Department of Genetics Laboratory of Imunopathology Keizo Asami), Amaral, Marcel C.O.(Federal University of Pernambuco Department of Genetics Laboratory of Imunopathology Keizo Asami), Araújo, Thiago A.S.(University of Pernambuco Institute of Biological Sciences ,University of Pernambuco Department of Exact and Natural Sciences), Fernandes, Késsia G.C.(University of Pernambuco Institute of Biological Sciences ,University of Pernambuco Department of Exact and Natural Sciences), Chaves, Adilson C.(University of Pernambuco Institute of Biological Sciences ,University of Pernambuco Department of Exact and Natural Sciences), Morais, Márcia M.C.(University of Pernambuco Institute of Biological Sciences)
• Format: Journal Article
• Language: English
• Published: São Paulo Sociedade Brasileira de Microbiologia 01-01-2007; Springer Nature B.V
• Subjects: aqueous two phase system; extracellular lipase; lipase extracelular; MICROBIOLOGY; PEG; sistema bifásico aquoso; Trichosporon; aqueous two phase system; PEG; extracellular lipase; lipase extracelular; sistema bifásico aquoso; Trichosporon
• ISSN: 1517-8382; 1678-4405; 1678-4405
• DOI: 10.1590/S1517-83822007000100013

A lipase-producing yeast strain isolated from crude cheese and identified as Trichosporon spp produced 7.3 U/mL (59.3 U/µg) after 72h of cultivation. Lipase showed optimum activity at pH 7.0-8.0 and 45-50ºC. Extraction by the two-phase aqueous system (PEG-phosphate salts) showed an elevated recuperation (99.8%) of enzymatic activity in the PEG phase.
Uma levedura produtora de lipase isolada de queijo coalho e identificada como Trichosporon spp produziu 7,3 U/mL (59,3 U/µg) após 72h de cultivo. A lipase mostrou atividade ótima em pH 7,0-8,0 e temperatura ótima entre 45-50ºC. Extração pelo sistema PEG - sais de fosfato apresentou 99,8% de recuperação da atividade enzimática na fase PEG.