Sensitive analysis of aminoglycoside antibiotics via hyphenation of transient moving substitution boundary with field-enhanced sample injection in capillary electrophoresis

• Published in: Journal of Chromatography A Vol. 1295; pp. 128 - 135
• Main Authors: Ge, Shuli, Tang, Wanrong, Han, Ruobing, Zhu, Yan, Wang, Qingjiang, He, Pingang, Fang, Yuzhi
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 21-06-2013; Elsevier
• Subjects: 18C6H4; Aminoglycoside antibiotics; Aminoglycosides - analysis; Amperometric detection; Analysis methods; Analytical chemistry; Anti-Bacterial Agents - analysis; Applied sciences; Capillary zone electrophoresis; Chemistry; Chromatographic methods and physical methods associated with chromatography; Electrophoresis, Capillary - methods; Exact sciences and technology; Field-enhanced sample injection; Freshwater; Moving substitution boundary; Natural water pollution; Other chromatographic methods; Pollution; Rivers - chemistry; Sensitivity and Specificity; Water Pollutants, Chemical - analysis; Water treatment and pollution; Moving substitution boundary; Aminoglycoside antibiotics; Amperometric detection; Capillary zone electrophoresis; 18C6H4; Field-enhanced sample injection; Solid phase extraction; Chemical analysis; Zone electrophoresis; Crown compound; Mobile phase; Mechanism; Chemical enrichment; Transients; Sample preparation; Sodium Hydroxides; Amperometry; Kanamycin; Stream; Trace analysis; Streptomycin; Phase composition; Capillary electrophoresis; Coupled method; Pseudostationary phase; Chemical substitution; Electrochemical detector; Organic solvent; Antibiotic; Aminoglycoside; Acetonitrile; Surface water; Neomycin; Emerging pollutant; Water pollution; Field enhanced sample injection; Antibacterial agent; River water
• ISSN: 0021-9673; 1873-3778
• DOI: 10.1016/j.chroma.2013.04.049

•A novel FESI-MSB method in CE-AD was developed.•The mechanism of MSB relied on substitution reaction between 18C6H4-AGs and Na+.•18C6H4 proved to be a novel PSP for analytes with primary amine groups focusing.•This strategy afforded about 400–1000-fold improvements in detection sensitivity.
A novel field-enhanced sample injection coupled with transient moving substitution boundary method in capillary electrophoresis was developed for aminoglycoside antibiotic (AG) analysis using 18-crown-6-tetracarboxylic acid (18C6H4) as a pseudostationary phase. Results indicated that the stacking mechanism of moving substitution boundary relied on the substitution reaction between 18C6H4-bonded AG complexes and Na+ at the substitution boundary. The stacking mechanism as well as important parameters governing pre-concentration and separation have been investigated in order to obtain maximum resolution and sensitivity. Under optimized conditions, using a sample prepared in a low-conductivity matrix, the limits of detection for streptomycin, neomycin, and kanamycin were 0.62, 5.9 and 8.6nM (S/N=3), respectively, and the detection sensitivities were improved 940-, 692-, and 415-fold, respectively. The method also gave accurate and reliable results in the analysis of AGs in river water samples.