Phylogenetic characterization of two novel species of the genus Bifidobacterium: Bifidobacterium saimiriisciurei sp. nov. and Bifidobacterium platyrrhinorum sp. nov

• Published in: Systematic and applied microbiology Vol. 43; no. 5; p. 126111
• Main Authors: Modesto, Monica, Satti, Maria, Watanabe, Koichi, Scarafile, Donatella, Huang, Chien-Hsun, Liou, Jong-Shian, Tamura, Tomohiko, Saito, Satomi, Watanabe, Mizuki, Mori, Koji, Huang, Lina, Sandri, Camillo, Spiezio, Caterina, Arita, Masanori, Mattarelli, Paola
• Format: Journal Article
• Language: English
• Published: Germany Elsevier GmbH 01-09-2020
• Subjects: Aldehyde-Lyases - metabolism; Animals; Bacterial Typing Techniques; Base Composition; Bifidobacterium; Bifidobacterium - classification; Bifidobacterium - genetics; Bifidobacterium - isolation & purification; Bifidobacterium - physiology; Bifidobacterium platyrrhinorumsp. nov; Bifidobacterium saimiriisciurei; Computer Simulation; Culture Media; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; Feces - microbiology; Genes, Bacterial; Genes, rRNA; Genetic Variation; Genome, Bacterial; Male; New species; Phylogeny; RNA, Ribosomal, 16S - genetics; Saimiri - microbiology; Saimiri sciureus; Sequence Analysis, DNA; Squirrel monkey; Temperature; Squirrel monkey; isDDH; Bifidobacterium; Bifidobacterium platyrrhinorumsp. nov; MLSA; New species; Bifidobacterium saimiriisciurei; Saimiri sciureus; ANI
• ISSN: 0723-2020; 1618-0984
• DOI: 10.1016/j.syapm.2020.126111

Three bifidobacterial Gram-stain-positive, non-spore forming and fructose-6-phosphate phosphoketolase-positive strains, SMA1T, SMB2 and SMA15T were isolated from the faeces of two adult males of the squirrel monkey (Saimiri sciureus).
On the basis of 16S rRNA gene sequence similarities, the type strain of Bifidobacterium primatium DSM 100687T (99.3%; similarity) was the closest neighbour to strains SMA1T and SMB2, whereas the type strain of Bifidobacterium stellenboschense DSM 23968T (96.5%) was the closest neighbour to strain SMA15T. The average nucleotide identity (ANI) values of SMA1T and SAM15T with the closely related type strains were 93.7% and 88.1%, respectively. The in silico DNA‒DNA hybridization values with the closest neighbours were 53.1% and 36.9%, respectively. GC contents of strains SMA1T and SMA15T were 63.6 and 66.4 mol%, respectively. Based on the phylogenetic, genotypic and phenotypic data obtained, the strains SMA1T and SMA15T clearly represent two novel taxa within the genus Bifidobacterium for which the names Bifidobacterium saimiriisciurei sp. nov. (type strain SMA1T = BCRC 81223T = NBRC 114049T = DSM 106020T) and Bifidobacterium platyrrhinorum sp. nov. (type strain SMA15T = BCRC 81224T = NBRC 114051T = DSM 106029T) are proposed.