Assignment of the Human Nuclear Hormone Receptor, NUC1 (PPARD), to Chromosome 6p21.1–p21.2

• Published in: Genomics (San Diego, Calif.) Vol. 35; no. 3; pp. 637 - 638
• Main Authors: Yoshikawa, Takeo, Brkanac, Zoran, Dupont, Barbara R., Xing, Guo-Qiang, Leach, Robin J., Detera-Wadleigh, Sevilla D.
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 01-08-1996; Elsevier
• Subjects: Biological and medical sciences; Cell Line; Chromosome Mapping; Chromosomes, Human, Pair 6; Classical genetics, quantitative genetics, hybrids; Fundamental and applied biological sciences. Psychology; Genetics of eukaryotes. Biological and molecular evolution; Human; Humans; Hybrid Cells; In Situ Hybridization, Fluorescence; Nuclear Proteins - genetics; Receptors, Cell Surface - genetics; Receptors, Cytoplasmic and Nuclear - genetics; Transcription Factors - genetics; Genetic mapping; Human; Nuclear receptor; Gene; Chromosome C6; Transcription factor; Peroxisome proliferator; Hormonal receptor
• ISSN: 0888-7543; 1089-8646
• DOI: 10.1006/geno.1996.0417

Nuclear hormone receptors act as ligand-activated transcription factors, constituting a large gene family. One of the subfamilies includes the peroxisomal proliferator-activated receptors (PPAR). To date, three mammalian PPAR subtypes have been isolated and termed PPAR alpha , PPAR gamma , and NUC1 (human) or PPAR delta (mouse). Recently, we have cloned rat PPAR delta and demonstrated that it contains a 14 CGG triplet repeat on the 5'-untranslated region (5'-UTR). It is not known whether the human homolog, NUC1 (PPARD), contains the same repeat. Functionally, PPAR alpha and PPAR gamma have been shown to be activated by a variety of fatty acids and hypolipidemic compounds that induce peroxisome proliferation in the liver. Furthermore, prostaglandin J sub(2) has been found to be a natural PPAR gamma ligand. Human NUC1 and mouse PPAR delta are known to be activated by C18 unsaturated fatty acids. To explore a possible relationship of NUC1 to previously identified disease loci, we set out to determine its chromosomal location. We have mapped NUC1 to chromosome 6 by a PCR-based somatic cell hybrid panel (BIOS), using the forward primer (5'-CTGGCTGGAGTCTCAGAGCAC) spanning nt 2831-2851 and the reverse primer (5'-TGGGCACTTCCACCCAGAGTC), which is complementary to nt 2930-2950. Both primers were derived from the 3'-UTR of NUC1. To localize the gene more precisely, we conducted fluorescence in situ hybridization (FISH) according to the previously described method.