Thermophilic fermentative hydrogen production from xylose by Thermotoga neapolitana DSM 4359

• Published in: Renewable energy Vol. 37; no. 1; pp. 174 - 179
• Main Authors: Ngo, Tien Anh, Nguyen, Tra Huong, Bui, Ha Thi Viet
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 2012; Elsevier
• Subjects: Acetic acid; Alternative fuels. Production and utilization; Applied sciences; Batch culture; Biofuel production; Biohydrogen; Biological and medical sciences; biomass; Bioreactors; Biotechnology; blood serum; bottles; CSABR; Culture; Energy; Exact sciences and technology; Fermentation; Fuels; Fundamental and applied biological sciences. Psychology; Hydrogen; Hydrogen production; Industrial applications and implications. Economical aspects; lactic acid; metabolites; renewable energy sources; Serums; Thermotoga neapolitana; Xylose; Xylose; Batch culture; Biohydrogen; CSABR; Thermotoga neapolitana
• ISSN: 0960-1481; 1879-0682
• DOI: 10.1016/j.renene.2011.06.015

Biohydrogen production from xylose by
Thermotoga neapolitana was investigated in batch culture using serum bottles and a continuously stirred anaerobic bioreactor (CSABR). The effect of various xylose concentrations on growth and H
2 production were studied in small batch culture for highly efficient H
2 production. The highest hydrogen production of 32.1 ± 1.6 mmol-H
2/L and maximum biomass concentration of 959.63 ± 47.9 mg/L were obtained at initial xylose concentration of 5.0 g/L. To develop a large-scale biohydrogen production system as well as overcome the problems in small batch culture, a continuously stirred anaerobic bioreactor was tested on
T. neapolitana in both pH-uncontrolled batch culture and pH-controlled batch culture. The results showed that the production level of H
2 from fermentation in a pH-controlled batch culture was much higher than those from a pH-uncontrolled batch culture for H
2 production from xylose. The H
2 yield in a pH-controlled batch culture on xylose substrate was 2.22 ± 0.11 mol-H
2 mol
−1 xylose
consumed, which was nearly 1.2-fold higher than pH-uncontrolled batch cultures. In order to study the precise effect of a stable pH on hydrogen production, and metabolite pathway involved, cultures was conducted with pH-controlled at different levels ranging from 6.5 to 7.5. The maximum H
2 yield of 2.8 ± 0.14 mol-H
2 mol
−1 xylose
consumed was measured while the pH was maintained at 7.0. The acetic acid and lactic acid production were 2.98 ± 0.15 g/L and 0.36 ± 0.02 g/L, respectively.
► Biohydrogen fermentation by the hyperthermophile
Thermotoga neapolitana from xylose substrate was conducted in both serum bottles and a continuously stirred anaerobic bioreactor (CSABR). ► The optimization of initial substrate concentration for growth and H
2 production were determined in serum bottles. ► The Investigation of pH values were studied in the CSABR for highly efficient H
2 production. ► The successful investigation in this study may be a potential culture technique for hydrogen production systems from
Thermotoga neapolitana.