Circadian Protection against Reactive Oxygen Species Involves Changes in Daily Levels of the Manganese- and Iron-Containing Superoxide Dismutase Isoforms in Lingulodinium polyedrum

• Published in: Biological rhythm research Vol. 32; no. 4; pp. 439 - 448
• Main Authors: Okamoto, O.K., Colepicolo, P.
• Format: Journal Article
• Language: English
• Published: Taylor & Francis Group 01-10-2001
• Subjects: Lingulodinium polyedrum
• ISSN: 0929-1016; 1744-4179
• DOI: 10.1076/brhm.32.4.439.1331

A circadian rhythm in the total activity of superoxide dismutase (SOD; EC 1.15.1.1) from the unicellular alga Lingulodinium polyedrum is shown to be attributable to the mitochondrial MnSOD and chloroplastic FeSOD isoforms. Activity gels and labelling with polyclonal antibodies against pure CuZnSOD, MnSOD and FeSOD revealed a distinct circadian pattern in the abundance of the latter two isoforms, with peak values in early photophase 5 times greater than at the dark phase. However, no such changes were detected for the CuZnSOD isoform, which remained at constant levels over the 24-h light/dark cycle. These SOD isoforms might provide protection against damage from photochemically generated oxygen radicals, thus preventing subcellular oxidative stress.