Vitamin D metabolites influence expression of genes concerning cellular viability and function in insulin producing β-cells (INS1E)

• Published in: Gene Vol. 746; p. 144649
• Main Authors: Bornstedt, Mette E., Gjerlaugsen, Nina, Olstad, Ole Kristoffer, Berg, Jens Petter, Bredahl, May K., Thorsby, Per M.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01-07-2020
• Subjects: Animals; Apoptosis - drug effects; Apoptosis - genetics; Cell Line, Tumor; Cell Survival - drug effects; Gene expression; Gene Expression Regulation - drug effects; Glucose-stimulated insulin secretion (GSIS); Insulin Secretion - drug effects; Insulin-Secreting Cells - cytology; Insulin-Secreting Cells - metabolism; Rats; Vitamin D; Vitamin D - analogs & derivatives; Vitamin D - pharmacokinetics; Vitamin D - pharmacology; β-Cell; GEO; T2DM; Vitamin D; VDR; RT-PCR; GSIS; Glucose-stimulated insulin secretion (GSIS); INS1E; IPA; β-cell; RPMI; Gene expression; β-Cell
• ISSN: 0378-1119; 1879-0038
• DOI: 10.1016/j.gene.2020.144649

•25(OH)VitD and 1,25(OH)2VitD changed gene expression in pancreatic β-cells, INS1E.•Vit D mainly affected genes concerning β-cell function, viability and apoptosis.•Vit D may increase glucose-induced insulin secretion by altered gene expression.
Studies have shown that vitamin D can enhance glucose-stimulated insulin secretion (GSIS) and change the expression of genes in pancreatic β-cells. Still the mechanisms linking vitamin D and GSIS are unknown.
We used an established β-cell line, INS1E. INS1E cells were pre-treated with 10 nM 1,25(OH)2vitamin D or 10 nM 25(OH)vitamin D for 72 h and stimulated with 22 mM glucose for 60 min. RNA was extracted for gene expression analysis.
Expression of genes affecting viability, apoptosis and GSIS changed after pre-treatment with both 1,25(OH)2vitamin D and 25(OH)vitamin D in INS1E cells. Stimulation with glucose after pre-treatment of INS1E cells with 1,25(OH)2vitamin D resulted in 181 differentially expressed genes, whereas 526 genes were differentially expressed after pre-treatment with 25(OH)vitamin D.
Vitamin D metabolites may affect pancreatic β-cells and GSIS through changed gene expression for genes involved in β-cell function and viability.