Ion-exchange high-performance liquid chromatography of nucleotides and polypeptides on new types of ion-exchange sorbents, based on polystyrene-coated silicas

A novel type of ion exchanger was prepared by multipoint covalent binding of polystyrene chains onto the surface of porous silica followed by polymer-analogous modification of the bonded layer. Both anion and cation exchangers were synthesized and examined in the separation of nucleotides and protei...

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Bibliographic Details
Published in:	Journal of chromatography Vol. 548; no. 1-2; p. 207
Main Authors:	Kurganov, A A, Davankov, V A, Unger, K K
Format:	Journal Article
Language:	English
Published:	Netherlands 12-07-1991
Subjects:	Animals Cattle Chromatography, High Pressure Liquid - instrumentation Chromatography, Ion Exchange - instrumentation Humans Ion Exchange Resins - chemistry Nucleotides - isolation & purification Peptides - isolation & purification Polystyrenes - chemistry Silicon Dioxide - chemistry
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Summary:	A novel type of ion exchanger was prepared by multipoint covalent binding of polystyrene chains onto the surface of porous silica followed by polymer-analogous modification of the bonded layer. Both anion and cation exchangers were synthesized and examined in the separation of nucleotides and proteins. Rapid and efficient separation of basic polypeptides on strong anion exchangers and that of acidic polypeptides on strong cation exchangers could be achieved. For the separation of complete mixtures of polypeptides the application of zwitter-ionic ion exchangers can be recommended.
DOI:	10.1016/s0021-9673(01)88602-7