The Influence of Permafrost Microorganisms on Monocytes Differentiation In Vitro

• Published in: Bulletin of experimental biology and medicine Vol. 175; no. 3; pp. 362 - 366
• Main Authors: Petrov, S. A., Sukhovei, Yu.G., Kalenova, L. F., Kostolomova, E. G., Subbotin, A. M., Kastornov, A. A.
• Format: Journal Article
• Language: English
• Published: New York Springer US 01-07-2023; Springer; Springer Nature B.V
• Subjects: Bacillus; Bacillus megaterium; Biomedical and Life Sciences; Biomedicine; CD14 antigen; CD16 antigen; Cell Biology; Internal Medicine; Laboratory Medicine; Leukocytes (mononuclear); Metabolites; Microorganisms; Monocytes; Pathology; Peripheral blood; Permafrost; Strains (organisms); monocytes differentiation regulatory factors; permafrost fossil microorganisms; monocytes subpopulations; bacterial metabolites; monocytes differentiation
• ISSN: 0007-4888; 1573-8221; 1573-8221
• DOI: 10.1007/s10517-023-05868-1

Metabolites of fossil microorganisms of dispersed watered rocks that have passed into a frozen state (
Bacillus sp
. strains 2/09 and 9/08,
Bacillus megaterium
8-75) can modulate the differentiation activity of human peripheral blood monocytes into phenotypically different subpopulations when cultured
in vitro
for 7 days. This effect is largely determined by the type of metabolites that depends on the temperature of their production: cold (collected after bacterium culturing at 5°C), medium (at 22°C), and warm temperature (at 37°C). All three types of metabolites had a weak negative influence on the level of classical (CD14
hi
CD16
–
) monocytes and stimulated the differentiation of intermediate (CD14
+
CD16
+
) and non-classical (CD14
lo
CD16
+
) monocytes. The monocytes differentiation into the subpopulation of intermediate (CD14
+
CD16
+
) was stimulated to a greater extent by medium-temperature metabolites of the strain 8/75 and into the subpopulation of non-classical (CD14
lo
CD16
+
) monocytes by warm metabolites of the strains 8/75 and 2/09 throughout culturing of mononuclear cells (days 1, 3, 7). Bearing in mind the anti-infection activity of intermediate and non-classical monocytes, we can consider strains 8/75
Bacillus megaterium
and 2/09
Bacillus sp
. promising for their in-depth testing.