Survival of bifidobacteria administered to calves

• Published in: Folia microbiologica Vol. 55; no. 4; pp. 390 - 392
• Main Authors: Vlkova, E.,Ceska Zemedelska Univ., Prague (Czech Republic). Katedra Mikrobiologie, Vyzivy a Dietetiky, Grmanova, M.,Ceska Zemedelska Univ., Prague (Czech Republic). Katedra Mikrobiologie, Vyzivy a Dietetiky, Killer, J.,Akademie Ved, Libechov (Czech Republic). Ustav Zivocisne Fyziologie a Genetiky, Mrazek, J.,Akademie Ved, Libechov (Czech Republic). Ustav Zivocisne Fyziologie a Genetiky, Kopecny, J.,Akademie Ved, Libechov (Czech Republic). Ustav Zivocisne Fyziologie a Genetiky, Bunesova, V.,Ceska Zemedelska Univ., Prague (Czech Republic). Katedra Mikrobiologie, Vyzivy a Dietetiky, Rada, V.,Ceska Zemedelska Univ., Prague (Czech Republic). Katedra Mikrobiologie, Vyzivy a Dietetiky
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01-07-2010; Springer Nature B.V
• Subjects: Acetic acid; Animals; Anti-Bacterial Agents - pharmacology; ANTIBIOTICOS; ANTIBIOTICS; ANTIBIOTIQUE; Applied Microbiology; Bacteria; Bacteria - classification; Bacteria - isolation & purification; Bacterial Typing Techniques; BIFIDOBACTERIUM; Bifidobacterium - drug effects; Bifidobacterium - metabolism; Bifidobacterium - physiology; Bifidobacterium animalis; Biomedical and Life Sciences; CALVES; Cattle; Cattle - microbiology; Cluster Analysis; Colony Count, Microbial; Cultivation; CULTIVO IN VITRO; CULTURE IN VITRO; CULTURE MEDIA; Digestive system; DNA, Bacterial - genetics; E coli; Environmental Engineering/Biotechnology; ESCHERICHIA COLI; FAECES; FECES; Feces - microbiology; Gastrointestinal tract; Gastrointestinal Tract - microbiology; HECES; http://www.fao.org/aos/agrovoc#c_10204; http://www.fao.org/aos/agrovoc#c_1219; http://www.fao.org/aos/agrovoc#c_23898; http://www.fao.org/aos/agrovoc#c_24135; http://www.fao.org/aos/agrovoc#c_2772; http://www.fao.org/aos/agrovoc#c_33700; http://www.fao.org/aos/agrovoc#c_34094; http://www.fao.org/aos/agrovoc#c_4144; http://www.fao.org/aos/agrovoc#c_492; http://www.fao.org/aos/agrovoc#c_5736; http://www.fao.org/aos/agrovoc#c_7538; Immunology; In Situ Hybridization; IN VITRO CULTURE; LACTOBACILLUS; Life Sciences; MEDIO DE CULTIVO; MICRO-ORGANISME DU RUMEN; Microbial Sensitivity Tests; Microbial Viability; Microbiology; MICROORGANISMOS DEL RUMEN; MILIEU DE CULTURE; Phylogeny; Probiotics - administration & dosage; Probiotics - isolation & purification; RESISTANCE AUX PRODUITS CHIMIQUES; RESISTANCE TO CHEMICALS; RESISTENCIA A PRODUCTOS QUIMICOS; RUMEN MICROORGANISMS; SUPERVIVENCIA; SURVIE; SURVIVAL; Survival analysis; TERNERO; Time Factors; VEAU; Probiotic Treatment; Broiler Chicken; Mupirocin; Fecal Sample; Lactobacillus
• ISSN: 0015-5632; 1874-9356
• DOI: 10.1007/s12223-010-0066-x

Twenty-five bifidobacteria were isolated from feces of calves. Isolates were identified, and their functional properties and antimicrobial activity were determined. From 10 strains with suitable properties rifampicin-resistant mutants (RRBs) were prepared and mixture of RRBs was administered to 2-d-old calves. These strains were identified by sequencing as Bifidobacterium animalis ssp. animalis (6 strains), B. thermophilum (2 strains), B. choerinum (1 strain) and B. longum ssp. suis (1 strain). The control group was without probiotic treatment. Survival ability of administered bifidobacteria was monitored in fecal samples by cultivation on modified TPY agar supplemented with mupirocin, acetic acid, and rifampicin. Administered bifidobacteria survived in gastrointestinal tract of calves for at least 60 d. Other bacteria were also determined after cultivation using fluorescence in situ hybridization (FISH). Bifidobacteria and lactobacilli dominated in fecal microflora. Significantly lower amounts of E. coli and higher amounts of bifidobacteria and total anaerobes were found in the treated group relative to the control group.