Quantitative Assay of B. pertussis Lipopolysaccharide
We studied activity of Bordetella pertussis LPS in the LAL test. The mean activity of various series of LPS preparations obtained from B. pertussis cells ranged from 1,950,000 to 2,940,000 endotoxin units/μg (EU/μg). Activity of the LPS preparation obtained from the culture medium supernatant was si...
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| Published in: | Bulletin of experimental biology and medicine Vol. 172; no. 6; pp. 718 - 720 |
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| Abstract | We studied activity of
Bordetella pertussis
LPS in the LAL test. The mean activity of various series of LPS preparations obtained from
B. pertussis
cells ranged from 1,950,000 to 2,940,000 endotoxin units/μg (EU/μg). Activity of the LPS preparation obtained from the culture medium supernatant was significantly higher (4,640,000 EU/μg). Activity of the control standard
E. coli
055:B5 LPS was 19,500±500 EU/μg. These data indicate that activity of the obtained preparations of
B. pertussis
LPS in the LAL test is 100-200 times higher than activity of
E. coli
LPS used as a reference control. It was concluded that the results of the LAL test when assessing the permissible content of
B. pertussis
endotoxins require correction, probably by introducing a correction factor. |
|---|---|
| AbstractList | We studied activity of Bordetella pertussis LPS in the LAL test. The mean activity of various series of LPS preparations obtained from B. pertussis cells ranged from 1,950,000 to 2,940,000 endotoxin units/[micro]g (EU/[micro]g). Activity of the LPS preparation obtained from the culture medium supernatant was significantly higher (4,640,000 EU/[micro]g). Activity of the control standard E. coli 055:B5 LPS was 19,500[+ or -]500 EU/[micro]g. These data indicate that activity of the obtained preparations of B. pertussis LPS in the LAL test is 100-200 times higher than activity of E. coli LPS used as a reference control. It was concluded that the results of the LAL test when assessing the permissible content of B. pertussis endotoxins require correction, probably by introducing a correction factor. We studied activity of Bordetella pertussis LPS in the LAL test. The mean activity of various series of LPS preparations obtained from B. pertussis cells ranged from 1,950,000 to 2,940,000 endotoxin units/[micro]g (EU/[micro]g). Activity of the LPS preparation obtained from the culture medium supernatant was significantly higher (4,640,000 EU/[micro]g). Activity of the control standard E. coli 055:B5 LPS was 19,500[+ or -]500 EU/[micro]g. These data indicate that activity of the obtained preparations of B. pertussis LPS in the LAL test is 100-200 times higher than activity of E. coli LPS used as a reference control. It was concluded that the results of the LAL test when assessing the permissible content of B. pertussis endotoxins require correction, probably by introducing a correction factor. Key Words: pertussis; B. pertussis; lipopolysaccharide; LAL test We studied activity of Bordetella pertussis LPS in the LAL test. The mean activity of various series of LPS preparations obtained from B. pertussis cells ranged from 1,950,000 to 2,940,000 endotoxin units/μg (EU/μg). Activity of the LPS preparation obtained from the culture medium supernatant was significantly higher (4,640,000 EU/μg). Activity of the control standard E. coli 055:B5 LPS was 19,500±500 EU/μg. These data indicate that activity of the obtained preparations of B. pertussis LPS in the LAL test is 100-200 times higher than activity of E. coli LPS used as a reference control. It was concluded that the results of the LAL test when assessing the permissible content of B. pertussis endotoxins require correction, probably by introducing a correction factor. We studied activity of Bordetella pertussis LPS in the LAL test. The mean activity of various series of LPS preparations obtained from B. pertussis cells ranged from 1,950,000 to 2,940,000 endotoxin units/μg (EU/μg). Activity of the LPS preparation obtained from the culture medium supernatant was significantly higher (4,640,000 EU/μg). Activity of the control standard E. coli 055:B5 LPS was 19,500±500 EU/μg. These data indicate that activity of the obtained preparations of B. pertussis LPS in the LAL test is 100-200 times higher than activity of E. coli LPS used as a reference control. It was concluded that the results of the LAL test when assessing the permissible content of B. pertussis endotoxins require correction, probably by introducing a correction factor. |
| Audience | Academic |
| Author | Nazirov, M. R. Parfenova, O. K. Kukes, V. G. Sidorov, N. G. Poddubikov, A. V. |
| Author_xml | – sequence: 1 givenname: M. R. surname: Nazirov fullname: Nazirov, M. R. organization: I. I. Mechnikov Research Institute of Vaccines and Sera – sequence: 2 givenname: A. V. surname: Poddubikov fullname: Poddubikov, A. V. organization: I. I. Mechnikov Research Institute of Vaccines and Sera – sequence: 3 givenname: V. G. surname: Kukes fullname: Kukes, V. G. organization: Scientific Centre for Expert Evaluation of Medicinal Products, Ministry of the Health of the Russian Federation – sequence: 4 givenname: N. G. surname: Sidorov fullname: Sidorov, N. G. email: deel@yandex.ru organization: I. I. Mechnikov Research Institute of Vaccines and Sera – sequence: 5 givenname: O. K. surname: Parfenova fullname: Parfenova, O. K. organization: V. N. Orekhovich Research Institute of Biomedical Chemistry |
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| Cites_doi | 10.3390/ijms22031029 10.1128/IAI.73.11.7381-7389.2005 10.22627/2072-8107-2020-19-2-58-63 10.30895/2221-996X-2021-21-1-10-19 10.15789/2220-7619-2019-2-354-362 10.1016/j.coi.2019.03.006 10.24411/2305-3496-2018-12008 |
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| Keywords | lipopolysaccharide LAL test B. pertussis pertussis |
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| References | Kapil, Merkel (CR7) 2019; 59 CR2 Niedziela, Letowska, Lukasiewicz, Kaszowska, Czarnecka, Kenne, Lugowski (CR8) 2005; 73 Alekseeva, Perelygina, Kolyshkina (CR1) 2021; 21 CR4 Prutskova, Cherdantsev, Andreeva (CR5) 2018; 7 Basov, Tsvirkun, Gerasimova, Zekoreeva (CR3) 2019; 9 Cherdantsev, Prutskova, Kostinov (CR6) 2020; 19 Ucieklak, Koj, Niedziela (CR9) 2021; 22 5463_CR4 P Kapil (5463_CR7) 2019; 59 5463_CR2 AA Basov (5463_CR3) 2019; 9 T Niedziela (5463_CR8) 2005; 73 IA Alekseeva (5463_CR1) 2021; 21 EV Prutskova (5463_CR5) 2018; 7 K Ucieklak (5463_CR9) 2021; 22 AP Cherdantsev (5463_CR6) 2020; 19 |
| References_xml | – volume: 22 start-page: 1029 issue: 3 year: 2021 ident: CR9 article-title: Conserved structural features of core oligosaccharides among the lipopolysaccharides of respiratory pathogens from the genus Bordetella analyzed exclusively by NMR spectroscopy publication-title: Int. J. Mol. Sci. doi: 10.3390/ijms22031029 contributor: fullname: Niedziela – volume: 73 start-page: 7381 issue: 11 year: 2005 end-page: 7389 ident: CR8 article-title: Epitope of the vaccine-type Bordetella pertussis strain 186 lipooligosaccharide and antiendotoxin activity of antibodies directed against the terminal pentasaccharide-tetanus toxoid conjugate publication-title: Infect. Immun. doi: 10.1128/IAI.73.11.7381-7389.2005 contributor: fullname: Lugowski – volume: 19 start-page: 58 issue: 2 year: 2020 end-page: 63 ident: CR6 article-title: New features of pertussis vaccination publication-title: Detsk. Infekt. doi: 10.22627/2072-8107-2020-19-2-58-63 contributor: fullname: Kostinov – volume: 21 start-page: 10 issue: 1 year: 2021 end-page: 19 ident: CR1 article-title: Pertussis vaccines and the role of Bordetella pertussis lipooligosaccharide in the immune response to pertussis infection and vaccination publication-title: BIOpreparaty. Profilaktika, Diagnostilka, Lechenie. doi: 10.30895/2221-996X-2021-21-1-10-19 contributor: fullname: Kolyshkina – ident: CR4 – ident: CR2 – volume: 9 start-page: 354 issue: 2 year: 2019 end-page: 362 ident: CR3 article-title: The problem of pertussis in some regions of the world publication-title: Infektsiya Immunitet. doi: 10.15789/2220-7619-2019-2-354-362 contributor: fullname: Zekoreeva – volume: 59 start-page: 72 year: 2019 end-page: 78 ident: CR7 article-title: Pertussis vaccines and protective immunity publication-title: Curr. Opin. Immunol. doi: 10.1016/j.coi.2019.03.006 contributor: fullname: Merkel – volume: 7 start-page: 71 issue: 2 year: 2018 end-page: 77 ident: CR5 article-title: Vaccines and vaccination against pertussis of adults and children publication-title: Infekt. Bol: Novosti, Mneniya, Obuchenie. doi: 10.24411/2305-3496-2018-12008 contributor: fullname: Andreeva – ident: 5463_CR4 – ident: 5463_CR2 – volume: 9 start-page: 354 issue: 2 year: 2019 ident: 5463_CR3 publication-title: Infektsiya Immunitet. doi: 10.15789/2220-7619-2019-2-354-362 contributor: fullname: AA Basov – volume: 59 start-page: 72 year: 2019 ident: 5463_CR7 publication-title: Curr. Opin. Immunol. doi: 10.1016/j.coi.2019.03.006 contributor: fullname: P Kapil – volume: 7 start-page: 71 issue: 2 year: 2018 ident: 5463_CR5 publication-title: Infekt. Bol: Novosti, Mneniya, Obuchenie. doi: 10.24411/2305-3496-2018-12008 contributor: fullname: EV Prutskova – volume: 73 start-page: 7381 issue: 11 year: 2005 ident: 5463_CR8 publication-title: Infect. Immun. doi: 10.1128/IAI.73.11.7381-7389.2005 contributor: fullname: T Niedziela – volume: 21 start-page: 10 issue: 1 year: 2021 ident: 5463_CR1 publication-title: BIOpreparaty. Profilaktika, Diagnostilka, Lechenie. doi: 10.30895/2221-996X-2021-21-1-10-19 contributor: fullname: IA Alekseeva – volume: 22 start-page: 1029 issue: 3 year: 2021 ident: 5463_CR9 publication-title: Int. J. Mol. Sci. doi: 10.3390/ijms22031029 contributor: fullname: K Ucieklak – volume: 19 start-page: 58 issue: 2 year: 2020 ident: 5463_CR6 publication-title: Detsk. Infekt. doi: 10.22627/2072-8107-2020-19-2-58-63 contributor: fullname: AP Cherdantsev |
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| Snippet | We studied activity of
Bordetella pertussis
LPS in the LAL test. The mean activity of various series of LPS preparations obtained from
B. pertussis
cells... We studied activity of Bordetella pertussis LPS in the LAL test. The mean activity of various series of LPS preparations obtained from B. pertussis cells... |
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| SubjectTerms | Biomedical and Life Sciences Biomedicine Cell Biology Cell culture E coli Endotoxins Escherichia coli Internal Medicine Laboratory Medicine Lipopolysaccharides Pathology Pertussis Tetanus antitoxin Whooping cough |
| Title | Quantitative Assay of B. pertussis Lipopolysaccharide |
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