Quantitative Assay of B. pertussis Lipopolysaccharide

• Published in: Bulletin of experimental biology and medicine Vol. 172; no. 6; pp. 718 - 720
• Main Authors: Nazirov, M. R., Poddubikov, A. V., Kukes, V. G., Sidorov, N. G., Parfenova, O. K.
• Format: Journal Article
• Language: English
• Published: New York Springer US 01-04-2022; Springer; Springer Nature B.V
• Subjects: Biomedical and Life Sciences; Biomedicine; Cell Biology; Cell culture; E coli; Endotoxins; Escherichia coli; Internal Medicine; Laboratory Medicine; Lipopolysaccharides; Pathology; Pertussis; Tetanus antitoxin; Whooping cough; lipopolysaccharide; LAL test; B. pertussis; pertussis
• ISSN: 0007-4888; 1573-8221
• DOI: 10.1007/s10517-022-05463-w

We studied activity of
Bordetella pertussis
LPS in the LAL test. The mean activity of various series of LPS preparations obtained from
B. pertussis
cells ranged from 1,950,000 to 2,940,000 endotoxin units/μg (EU/μg). Activity of the LPS preparation obtained from the culture medium supernatant was significantly higher (4,640,000 EU/μg). Activity of the control standard
E. coli
055:B5 LPS was 19,500±500 EU/μg. These data indicate that activity of the obtained preparations of
B. pertussis
LPS in the LAL test is 100-200 times higher than activity of
E. coli
LPS used as a reference control. It was concluded that the results of the LAL test when assessing the permissible content of
B. pertussis
endotoxins require correction, probably by introducing a correction factor.