Undecanoic Acid, Lauric Acid, and N-Tridecanoic Acid Inhibit Escherichia coli Persistence and Biofilm Formation

• Published in: Journal of microbiology and biotechnology Vol. 31; no. 1; pp. 130 - 136
• Main Authors: Jin, Xing, Zhou, Jiacheng, Richey, Gabriella, Wang, Mengya, Hong, Sung Min Choi, Hong, Seok Hoon
• Format: Journal Article
• Language: English
• Published: Korea (South) 한국미생물·생명공학회 28-01-2021
• Subjects: Anti-Bacterial Agents - pharmacology; Bacterial Infections - drug therapy; Biofilms - drug effects; Biofilms - growth & development; Drug Resistance, Bacterial; Escherichia coli - drug effects; Fatty Acids - pharmacology; Humans; Lauric Acids - pharmacology; 생물학; medium-chain saturated fatty acids; biofilms; E. coli; persister cells
• ISSN: 1017-7825; 1738-8872
• DOI: 10.4014/jmb.2008.08027

Persister cell formation and biofilms of pathogens are extensively involved in the development of chronic infectious diseases. Eradicating persister cells is challenging, owing to their tolerance to conventional antibiotics, which cannot kill cells in a metabolically dormant state. A high frequency of persisters in biofilms makes inactivating biofilm cells more difficult, because the biofilm matrix inhibits antibiotic penetration. Fatty acids may be promising candidates as antipersister or antibiofilm agents, because some fatty acids exhibit antimicrobial effects. We previously reported that fatty acid ethyl esters effectively inhibit
persister formation by regulating an antitoxin. In this study, we screened a fatty acid library consisting of 65 different fatty acid molecules for altered persister formation. We found that undecanoic acid, lauric acid, and N-tridecanoic acid inhibited
BW25113 persister cell formation by 25-, 58-, and 44-fold, respectively. Similarly, these fatty acids repressed persisters of enterohemorrhagic
EDL933. These fatty acids were all medium-chain saturated forms. Furthermore, the fatty acids repressed Enterohemorrhagic
(EHEC) biofilm formation (for example, by 8-fold for lauric acid) without having antimicrobial activity. This study demonstrates that medium-chain saturated fatty acids can serve as antipersister and antibiofilm agents that may be applied to treat bacterial infections.