Replication and immunoactivity of the recombinant Peromyscus maniculatus cytomegalovirus expressing hantavirus G1 glycoprotein in vivo and in vitro

• Published in: Vaccine Vol. 24; no. 3; pp. 327 - 334
• Main Authors: Rizvanov, Albert A., Khaiboullina, Svetlana F., van Geelen, Albert G.M., St. Jeor, Stephen C.
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 16-01-2006; Elsevier
• Subjects: Animals; Applied microbiology; Biological and medical sciences; Cytomegalovirus; Cytomegalovirus - genetics; Cytomegalovirus - immunology; DNA - biosynthesis; DNA - genetics; DNA Replication; Fundamental and applied biological sciences. Psychology; Hantavirus; Hantavirus - genetics; Hantavirus - immunology; Microbiology; Miscellaneous; Peromyscus - virology; Peromyscus maniculatus; Recombinant virus; Reverse Transcriptase Polymerase Chain Reaction; Sin Nombre; Sin Nombre virus; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects); Vaccines, Synthetic - immunology; Viral Envelope Proteins - biosynthesis; Viral Envelope Proteins - genetics; Viral Vaccines - immunology; Virology; Virus Replication; Whole-Body Irradiation; Cytomegalovirus; Peromyscus maniculatus; Sin Nombre; Hantavirus; Recombinant virus; Herpesviridae; Rodentia; Glycoprotein; Betaherpesvirinae; Infection; Virus; Vertebrata; Mammalia; Bunyaviridae; Viral disease; Replication
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/j.vaccine.2005.07.070

Previously, we have shown that CMV isolated from deer mouse could be used in vivo and in vitro to express Sin Nombre virus (SNV) glycoprotein G1 in deer mice [Rizvanov AA, van Geelen AG, Morzunov S, et al. Generation of a recombinant cytomegalovirus for expression of a hantavirus glycoprotein. J.Virol. 2003;77(22):12203–10]. In this report, we further characterize replication of wild-type (wt) and recombinant
Peromyscus CMV (PCMV) in vivo and in vitro using realtime PCR, and infectious center assays. Our findings indicate that both wt PCMV and recombinant PCMV establish persistent infections in
P. maniculatus. In addition, we demonstrated that gamma irradiation of PCMV infected mice resulted in reactivation of recombinant PCMV in persistently infected mice.