Isolation and primary structure of two peptides with cardioacceleratory and hyperglycemic activity from the corpora cardiaca of Periplaneta americana

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 81; no. 17; pp. 5575 - 5579
• Main Authors: Scarborough, R.M, Jamieson, G.C, Kalish, F, Kramer, S.J, McEnroe, G.A, Miller, C.A, Schooley, D.A
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences of the United States of America 01-09-1984; National Acad Sciences
• Subjects: Amino Acid Sequence; Amino acids; Animals; Bioassay; Chemical composition; Chromatography, High Pressure Liquid; Cockroaches; Corpora cardiaca; Glycemic effects; Grasshoppers; Heart Rate - drug effects; Hemolymph; Hyperglycemia - chemically induced; Insect biochemistry; Invertebrates; ISOLATION TECHNIQUE; Molecular Weight; Neurotransmitter agents; PEPTIDES; Peptides - isolation & purification; Peptides - pharmacology; Peptides - toxicity; PERIPLANETA AMERICANA; STRUCTURE
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.81.17.5575

Two cardioacceleratory peptides from the corpora cardiaca of Periplaneta americana have been purified by gel filtration and reversed-phase liquid chromatography. Based on analysis of the intact factors and their chymotryptic fragments, we have assigned the primary structure of these octapeptides as pGlu-Val-Asn-Phe-Ser-Pro-Asn-Trp-NH2, designated periplanetin CC-1, and pGlu-Leu-Thr-Phe-Thr-Pro-Asn-Trp-NH2, designated periplanetin CC-2. They represent new members of a family of invertebrate peptides that includes locust adipokinetic hormone and crustacean red-pigment concentrating hormone. Both peptides show adipokinetic activity in grasshoppers and hyperglycemic activity in cockroaches. One of these peptides (CC-2) has provocative sequence homology with the NH2-terminal portion of glucagon.