Genetic analysis of poliovirus strains isolated from sewage in Poland

• Published in: Journal of medical virology Vol. 86; no. 7; pp. 1243 - 1248
• Main Authors: Kuryk, Ł., Wieczorek, M., Diedrich, S., Böttcher, S., Witek, A., Litwińska, B.
• Format: Journal Article
• Language: English
• Published: United States Blackwell Publishing Ltd 01-07-2014; Wiley Subscription Services, Inc
• Subjects: 5' Untranslated Regions; Capsid Proteins - genetics; environmental surveillance; Genetics; Humans; Mutation; Neutralization Tests; OPV; Poland; polio eradication; Poliomyelitis; Poliovirus - classification; Poliovirus - genetics; Poliovirus - isolation & purification; recombinant; Recombination, Genetic; RNA Replicase - genetics; RNA, Viral - genetics; Sequence Analysis, DNA; sequencing; Serotyping; Sewage - virology; Virology; Waste materials; Poland; recombinant; OPV; environmental surveillance; polio eradication; sequencing
• ISSN: 0146-6615; 1096-9071
• DOI: 10.1002/jmv.23803

The study describes genetic characterization of poliovirus (PV) strains isolated from sewage samples in Poland. The analyses were performed for the detection of any putative polio revertants and recombinants in three genomic regions by sequencing analysis. Thirty‐six strains were analyzed. The analyzed strains were identified by neutralization assay as 7 strains of serotype P1, 10 strains of serotype P2, and 19 strains of serotype P3. Sewage isolates were sequenced in 5′UTR, VP1, and 3D genomic regions. All detected PVs were classified as vaccine strains on the basis of VP1 sequence. Mutational differences in the VP1 sequences of isolated viruses ranged from 0.0% to 0.4%, indicating a limited replication period. The genetic analysis of the 3D region showed that some strains have recombinant genomes. Nine strains were found as dipartite recombinants (seven strains—S3/S2, one strain—S2/S1, one strain—S3/S1), while one strain was found as tripartite recombinant (S3/S2/S1). No recombinants with non‐PV enteroviruses were identified. None of wild‐type PVs or vaccine‐derived polioviruses (VDPVs) were detected. This study showed the absence of wild or VDPV circulation in the country and demonstrated the usefulness of environmental surveillance in addition to acute flaccid paralysis (AFP) surveillance in support of polio eradication initiatives. J. Med. Virol. 86:1243–1248, 2014. © 2013 Wiley Periodicals, Inc.