Synthesis, docking and characterization of some novel 5-(S-alkyl)-1.3.4-thiadiazole-2-carboxamide derivatives as anti-inflammatory and antibacterial agents

• Published in: BMC chemistry Vol. 18; no. 1; pp. 138 - 21
• Main Authors: El-Saghier, Ahmed M., Abdul-Baset, Asmaa, El-Hady, Omer M., El-Raheem, Walaa M. Abd, Kadry, Asmaa M.
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 27-07-2024; BioMed Central Ltd; Springer Nature B.V; BMC
• Subjects: 1,3,4-thiadiazole; Albumin; Aldehydes; Alkylation; Anti-inflammatory agent; Antiinfectives and antibacterials; Antimicrobial; Bacteria; Biopolymer denaturation; Carboxamide; Chemistry; Chemistry and Materials Science; Chemistry/Food Science; Ciprofloxacin; Coliforms; Denaturation; Diclofenac; E coli; Escherichia coli; Gram-positive bacteria; Methicillin; Molecular docking; Nonsteroidal anti-inflammatory drugs; Proteins; Reagents; Serum albumin; Sodium; Spectrum analysis; Thiadiazoles; Thiohydrazides; Thioxoacetamide; Thioxoacetamide; Antimicrobial; Carboxamide; 1,3,4-thiadiazole; Anti-inflammatory agent; Thiohydrazides
• ISSN: 2661-801X; 2661-801X
• DOI: 10.1186/s13065-024-01237-9

Because of the great pharmacological and industrial significance of 1,3,4-thiadiazole and its related compounds, researchers are still very interested in them. For this reason, in this study, we looked at ways to create new hybrid compounds containing carboxamide and 1,3,4-thiadiazole moieties. The thioxoacetamide derivatives used to make these compounds were reacted with various alkylated reagents to produce multiple
S
-alkyl groups. Additionally, these compounds were reacted with aldehydes to form novel derivatives known as 5-(substituent)-
N
-phenyl-1,3,4-thiadiazole-2-carboxamide. Here, we used the agar well diffusion method to examine the antibacterial activity of all the produced compounds against a few pathogenic bacteria that were resistant to multiple drugs. Additionally, look into their capacity to lower inflammation through the use of bovine serum albumin in the protein denaturation procedure. The substances were characterized by spectral analysis (IR,
1
HNMR,
13
CNMR and Elemental Analysis), and efficient as antibacterial agents against all the tested bacterial strains, except for
Escherichia coli
. Compounds
4a
and
8c
showed the highest level of inhibition zone against Gram-positive bacteria (
Staph. aureus, Bacillus subtilis
) at concentration 0.3, 0.4 and 0.5 mg/ml compared with ciprofloxacin at the same concentrations. The results demonstrated that every compound has significant anti-inflammatory activity. At a concentration of 250 µg/ml, compounds
3a
,
4c
and
8c
had the highest percentage inhibition of protein denaturation when (83.24%, 86.44% and 85.14%, respectively) compared to other compounds and diclofenac sodium as reference drug. Comparing compounds
4c
and
8c
to ciprofloxacin and diclofenac sodium, they showed powerful antibacterial and anti-inflammatory action. Furthermore, an investigation using molecular docking against DHPS from
S. aureus
(PDB ID: 6CLV) showed a strong connection with the intended protein and an elevated docking score, making it a prime candidate for antibiotics.