Hydroxyapatite and a New Fibrin Sealant Derived from Snake Venom as Scaffold to Treatment of Cranial Defects in Rats

• Published in: Materials research (São Carlos, São Paulo, Brazil) Vol. 18; no. 1; pp. 196 - 203
• Main Authors: Cunha, Marcelo Rodrigues da, Menezes, Fernanda Araujo, Santos, Geovane Ribeiro dos, Pinto, Clóvis Antônio Lopes, Barraviera, Benedito, Martins, Virginia da Conceição Amaro, Plepis, Ana Maria de Guzzi, Ferreira Junior, Rui Seabra
• Format: Journal Article
• Language: English
• Published: ABM, ABC, ABPol 01-02-2015; Associação Brasileira de Metalurgia e Materiais (ABM); Associação Brasileira de Cerâmica (ABC); Associação Brasileira de Polímeros (ABPol)
• Subjects: bioceramic; Biomedical materials; bone regeneration; Bones; Defects; ENGINEERING, CHEMICAL; Fibrin; fibrin sealant; Grafting; Hydroxyapatite; MATERIALS SCIENCE, MULTIDISCIPLINARY; METALLURGY & METALLURGICAL ENGINEERING; Sealers; Surgical implants; bioceramic; bone regeneration; fibrin sealant
• ISSN: 1516-1439; 1980-5373; 1516-1439; 1980-5373
• DOI: 10.1590/1516-1439.316014

Biomaterials are used as a promising alternative to bone grafts, including bioceramics whose composition resembles that of bone and fibrin sealants due to their hemostatic properties. The objective was to evaluate the repair of cranial defects in 40 rats, grafted with hydroxyapatite and a new fibrin sealant derived from snake venom. The animals were divided into four groups: C (control, no graft); Ha (hydroxyapatite); FS (fibrin sealant), and HaFS (hydroxyapatite and fibrin sealant). The animals were euthanized 2 and 6 weeks after surgery and wound area were submitted to analysis. After 2 weeks, immature bone was formed from the borders of the defect and in groups Ha and HaFS, few hydroxyapatite particles were surrounded by new bone. After 6 weeks, the new bone was mature and surrounded several hydroxyapatite particles, without connective tissue interposition and the volume of new bone was higher in HaFS group. The hydroxyapatite in combination with the new fibrin sealant accelerates bone repair.