Phytochemical profile and angiotensin I converting enzyme (ACE) inhibitory activity of Limonium michelsonii Lincz

• Published in: Journal of natural medicines Vol. 71; no. 4; pp. 650 - 658
• Main Authors: Jenis, Janar, Kim, Jeong Yoon, Uddin, Zia, Song, Yeong Hun, Lee, Hyeong-Hwan, Park, Ki Hun
• Format: Journal Article
• Language: English
• Published: Singapore Springer Singapore 01-10-2017; Springer Nature B.V
• Subjects: Aglycones; Angiotensin; Angiotensin I; Angiotensin-Converting Enzyme Inhibitors - isolation & purification; Angiotensin-Converting Enzyme Inhibitors - pharmacology; Animals; Biomedical and Life Sciences; Biomedicine; Blood circulation; Complementary & Alternative Medicine; Enzymes; Flavones; Flavonoids; Flavonoids - isolation & purification; Flavonoids - pharmacology; Flavonols; Glycosides; Glycosides - isolation & purification; Glycosides - pharmacology; Herbal medicine; Limonium; Lung - metabolism; Medicinal Chemistry; Medicinal plants; Metabolites; Original Paper; Peptidyl-Dipeptidase A - metabolism; Pharmacology/Toxicology; Pharmacy; Phenolic compounds; Phenols - isolation & purification; Phenols - pharmacology; Phytochemicals; Plant Extracts - chemistry; Plant Extracts - pharmacology; Plant Sciences; Plants, Medicinal - chemistry; Plumbaginaceae - chemistry; Quercetin; Rabbits; Secondary metabolites; Lincz; Competitive inhibitors; Angiotensin I converting enzyme; Phenolic metabolites; Limonium michelsonii Lincz
• ISSN: 1340-3443; 1861-0293
• DOI: 10.1007/s11418-017-1095-4

Members of the genus
Limonium
are widely used as medicinal herbs due to their health-promoting effects, such as an ability to improve blood circulation by inhibiting angiotensin I converting enzyme (ACE). While the potential of
L. michelsonii
Lincz. (a medicinal plant endemic to Kazakhstan) to inhibit ACE has been demonstrated, the inhibitory activities of its secondary metabolites have not been explored. In this work, the principal phenolic compounds (
1
–
20
) among these metabolites were isolated to determine the components responsible for ACE inhibition. The natural abundances of the active constituents within the target plant were characterized by UPLC-Q-TOF/MS analysis. All of the isolated compounds except for gallates
10
–
12
were found to significantly inhibit ACE, with IC
50
values of between 7.1 and 138.4 μM. Unexpectedly, the flavonol glycosides
16
–
20
were observed to be more potent than the corresponding aglycones
4
and
5
. For example, quercetin (
4
) had IC
50
 = 30.3 μM, whereas its glycosides (
16
,
17
) had IC
50
 = 10.2 and 14.5 μM, respectively. A similar trend was observed for myricetin (
5
) and its glycosides (
18
–
20
). In a kinetic study, the flavonols
3
–
5
and
16
–
20
and the dihydroflavonols
8
and
9
behaved as competitive inhibitors, whereas other flavones (
1
,
2
,
13
–
15
) and flavanones (
6
,
7
) performed noncompetitive inhibition.