An enzyme logic bioprotonic transducer

• Published in: APL materials Vol. 3; no. 1; pp. 014906 - 014906-6
• Main Authors: Miyake, Takeo, Josberger, Erik E., Keene, Scott, Deng, Yingxin, Rolandi, Marco
• Format: Journal Article
• Language: English
• Published: United States American Institute of Physics 01-01-2015; AIP Publishing LLC
• ISSN: 2166-532X; 2166-532X
• DOI: 10.1063/1.4900886

Translating ionic currents into measureable electronic signals is essential for the integration of bioelectronic devices with biological systems. We demonstrate the use of a Pd/PdHx electrode as a bioprotonic transducer that connects H+ currents in solution into an electronic signal. This transducer exploits the reversible formation of PdHx in solution according to PdH↔Pd + H+ + e−, and the dependence of this formation on solution pH and applied potential. We integrate the protonic transducer with glucose dehydrogenase as an enzymatic and gate for glucose and NAD+. PdHx formation and associated electronic current monitors the output drop in pH, thus transducing a biological function into a measurable electronic output.