Use of xylosidase 3C from Segatella baroniae to discriminate xylan non-reducing terminus substitution characteristics

• Published in: BMC research notes Vol. 17; no. 1; pp. 175 - 9
• Main Authors: St John, Franz J, Bynum, Loreen, Tauscheck, Dante A, Crooks, Casey
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 24-06-2024; BioMed Central; BMC
• Subjects: Amino acids; Arabinose - analogs & derivatives; Chromatography; Cloning; Comparative analysis; Enzymes; Genomics; GH3; Glucuronates - metabolism; Glycoside hydrolase; Glycoside hydrolase family 3; Hydrolases; Identification and classification; Laboratory methods; Microbial enzymes; Monosaccharides; Oligosaccharides; Oligosaccharides - chemistry; Oligosaccharides - metabolism; Prevotella - enzymology; Prevotella - genetics; Prevotella bryantii; Protein expression; Proteins; Segatella baroniae; Sodium; Structure; Substrate Specificity; Sugars; Xylan; Xylans - metabolism; Xylooligosaccharides; Xylose; Xylose - metabolism; Xylosidase; Xylosidases - chemistry; Xylosidases - genetics; Xylosidases - metabolism; United States; GH3; Xylosidase; Xylooligosaccharides; Segatella baroniae; Prevotella bryantii; XOS; Glycoside hydrolase family 3
• ISSN: 1756-0500; 1756-0500
• DOI: 10.1186/s13104-024-06835-3

New characterized carbohydrate-active enzymes are needed for use as tools to discriminate complex carbohydrate structural features. Fungal glycoside hydrolase family 3 (GH3) β-xylosidases have been shown to be useful for the structural elucidation of glucuronic acid (GlcA) and arabinofuranose (Araf) substituted oligoxylosides. A homolog of these GH3 fungal enzymes from the bacterium Segatella baroniae (basonym Prevotella bryantii), Xyl3C, has been previously characterized, but those studies did not address important functional specificity features. In an interest to utilize this enzyme for laboratory methods intended to discriminate the structure of the non-reducing terminus of substituted xylooligosaccharides, we have further characterized this GH3 xylosidase.
In addition to verification of basic functional characteristics of this xylosidase we have determined its mode of action as it relates to non-reducing end xylose release from GlcA and Araf substituted oligoxylosides. Xyl3C cleaves xylose from the non-reducing terminus of β-1,4-xylan until occurrence of a penultimate substituted xylose. If this substitution is O2 linked, then Xyl3C removes the non-reducing xylose to leave the substituted xylose as the new non-reducing terminus. However, if the substitution is O3 linked, Xyl3C does not hydrolyze, thus leaving the substitution one-xylose (penultimate) from the non-reducing terminus. Hence, Xyl3C enables discrimination between O2 and O3 linked substitutions on the xylose penultimate to the non-reducing end. These findings are contrasted using a homologous enzyme also from S. baroniae, Xyl3B, which is found to yield a penultimate substituted nonreducing terminus regardless of which GlcA or Araf substitution exists.