Development of an efficient plant dual cytosine and adenine editor

• Published in: Journal of integrative plant biology Vol. 63; no. 9; pp. 1600 - 1605
• Main Authors: Xu, Rongfang, Kong, Fanna, Qin, Ruiying, Li, Juan, Liu, Xiaoshuang, Wei, Pengcheng
• Format: Journal Article
• Language: English
• Published: China (Republic : 1949- ) Wiley Subscription Services, Inc 01-09-2021; Key Laboratory of Rice Genetic Breeding of Anhui Province,Rice Research Institute,Anhui Academy of Agricultural Sciences,Hefei 230031,China%Key Laboratory of Rice Genetic Breeding of Anhui Province,Rice Research Institute,Anhui Academy of Agricultural Sciences,Hefei 230031,China; College of Agronomy,Anhui Agricultural University,Hefei 230036,China
• Subjects: Adenine; Adenine - metabolism; Animals; base editing; CRISPR; Cytidine Deaminase - metabolism; Cytosine; dual‐base editor; Editing; Gene Editing - methods; genome editing; Genome, Plant; Genomes; Lampreys - genetics; Oryza; Plant breeding; Plant cells; Plants, Genetically Modified; rice; CRISPR; genome editing; rice; base editing; dual-base editor
• ISSN: 1672-9072; 1744-7909
• DOI: 10.1111/jipb.13146

Summary
An enhanced CDA‐like (eCDAL) was established from Japanese lamprey CDA1‐like 4 to achieve a high editing frequency in a broad region as a C‐terminal cytosine base editors (CT‐CBE). Then, a novel plant dual‐base editor version 1(pDuBE1) was developed by integrating TadA‐8e into eCDAL. The editing efficiency of pDuBE1 could reach to 87.6%, with frequencies of concurrent A‐to‐G and C‐to‐T conversions as high as 49.7% in stably transformed plant cells. Our results showed that pDuBE1 could mediate robust dual editing in plant genome, providing a powerful manipulation tool for precise crop breeding and screening platforms for in planta direct evolution.
The plant Dual Base Editor 1 (pDuBE1) system can simultaneously edit cytosine and adenine bases with robust activity, thus providing a powerful tool for precise crop breeding and screening platforms for in planta direct evolution.