A potentiometric non-enzymatic glucose sensor using a molecularly imprinted layer bonded on a conducting polymer

• Published in: Biosensors & bioelectronics Vol. 91; pp. 276 - 283
• Main Authors: Kim, Dong-Min, Moon, Jong-Min, Lee, Won-Chul, Yoon, Jang-Hee, Choi, Cheol Soo, Shim, Yoon-Bo
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 15-05-2017
• Subjects: Acrylamides - chemistry; Aminophenyl boronic acid; Aniline Compounds - chemistry; Biosensing Techniques - methods; Blood Glucose - analysis; Boronic Acids - chemistry; Conducting polymer; Electrodes; Glucose; Glucose - analysis; Gold - chemistry; Humans; Limit of Detection; Metal Nanoparticles - chemistry; Molecular Imprinting - methods; Molecularly imprinted polymer; Polymers - chemistry; Potentiometric sensor; Potentiometry - methods; Reproducibility of Results; Saliva - chemistry; Thiophenes - chemistry; Aminophenyl boronic acid; Potentiometric sensor; Glucose; Conducting polymer; Molecularly imprinted polymer
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/j.bios.2016.12.046

A non-enzymatic potentiometric glucose sensor for the determination of glucose in the micomolar level in saliva was developed based on a molecularly imprinted polymer (MIP) binding on a conducting polymer layer. A MIP containing acrylamide, and aminophenyl boronic acid, as a host molecule to glucose, was immobilized on benzoic acid-functionalized poly(terthiophene) (pTBA) by the amide bond formation onto a gold nanoparticles deposited-screen printed carbon electrode (pTBA/AuNPs/SPCE). Aromatic boronic acid was incorporated into the MIP layer to stably capture glucose and create a potentiometric signal through the changed pKa value of polymer film by the formation of boronate anion-glucose complex with generation of H+ ions by the cis-diol reaction. Reversible binding and extraction of glucose on the sensor surface was observed using a quartz crystal microbalance. Each layer of the sensor probe was characterized by cyclic voltammetry, electrochemical impedance spectroscopy, X-ray photoelectron spectroscopy, and atomic force microscopy. The potentiometric response at the optimized conditions exhibited a wide linear dynamic range of 3.2×10−7 to 1.0×10−3M, with a detection limit of 1.9 (±0.15)×10−7M. The sensor probe revealed an excellent selectivity and sensitivity for glucose compared to other saccharides. In addition, the reliability of the proposed glucose sensor was evaluated in physiological fluid samples of saliva and finger prick blood.
•A non-enzymatic potentiometric glucose sensor using a MIP is successfully developed.•The sensing performance of MIP is improved by its chemical bonding on a conducting polymer.•The proposed sensor is successfully applied for physiological fluid samples of saliva and blood.