Chromosome-scale genome assembly of Astragalus membranaceus using PacBio and Hi-C technologies

• Published in: Scientific data Vol. 11; no. 1; pp. 1071 - 11
• Main Authors: Fan, Huijie, Chai, Zhi, Yang, Xukui, Liu, Ake, Sun, Haifeng, Wu, Zhangyan, Li, Qingshan, Ma, Cungen, Zhou, Ran
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 02-10-2024; Nature Publishing Group; Nature Portfolio
• Subjects: 631/208/212; 631/449/2491; Astragalus membranaceus; Astragalus propinquus - genetics; Biosynthesis; Chromosomes; Chromosomes, Plant; Data Descriptor; Flavonoids; Genes; Genome, Plant; Genomes; Genomics; Herbal medicine; Humanities and Social Sciences; Leguminosae; Long terminal repeat; Medicinal plants; Molecular Sequence Annotation; multidisciplinary; Plants, Medicinal - genetics; Proteins; Science; Science (multidisciplinary); Software; Traditional Chinese medicine; Transcription factors
• ISSN: 2052-4463; 2052-4463
• DOI: 10.1038/s41597-024-03852-6

Astragalus membranaceus
(Fisch.) Bge (AM) is a medicinal herb plant belonging to the Leguminosae family. In this study, we present a chromosome-scale genome assembly of AM, aiming to enhance the molecular biology and functional studies of Astragali Radix. The genome size of AM is about 1.43 Gb, with a contig N50 value of 1.67 Mb. A total of 98.16% of the assembly anchored to 9 pseudochromosomes using Hi-C technology. The assembly completeness was estimated to be 97.27% using BUSCO with the long terminal repeat assembly index (LAI) of 16.22 and quality value (QV) of 48.58. Additionally, the genome contained 67.98% repetitive sequences. Genome annotation predicted 29,914 protein-coding genes, including 73 genes involved in the flavonoid biosynthetic pathway and 2,048 transcription factors. The high-quality genome assembly and gene annotation resources will greatly facilitate future functional genomic studies in Leguminosae species.