Radical peroxidation of palmitoyl-lineloyl-glycerophosphocholine liposomes: Identification of long-chain oxidised products by liquid chromatography–tandem mass spectrometry

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Vol. 855; no. 2; pp. 186 - 199
• Main Authors: Reis, Ana, Domingues, M.R.M., Amado, F.M.L., Ferrer-Correia, A.J., Domingues, P.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 15-08-2007; Elsevier Science
• Subjects: Analysis; Analytical, structural and metabolic biochemistry; Biological and medical sciences; Chromatography, Liquid - methods; Fatty Acids - analysis; Fatty Acids - chemistry; Fundamental and applied biological sciences. Psychology; General pharmacology; Glycerophosphatidylcholine; Glycerylphosphorylcholine - chemistry; LC–MS/MS; Lipid Peroxidation; Liposomes; Medical sciences; Oxidation; Pharmacology. Drug treatments; Phosphatidylcholines - analysis; Phosphatidylcholines - chemistry; Phospholipids; Tandem Mass Spectrometry - methods; Phospholipids; Oxidation; Glycerophosphatidylcholine; LC–MS/MS; Long chain; Liposome; Phospholipid; Liquid chromatography; Complex lipid; Metabolism; LC-MS/MS; Phosphatidylcholine; Liquid product; Qualitative analysis; Pharmacokinetics; Peroxidation
• ISSN: 1570-0232; 1873-376X
• DOI: 10.1016/j.jchromb.2007.05.006

Liquid chromatography coupled with electrospray tandem mass spectrometry (LC–MS/MS) was used to identify palmitoyl-lineloyl-glycerophosphatidylcholine oxidation products (PL(O
1–6)PC). Structural and positional isomers of keto, hydroxy and/or epoxy, and hydroperoxide derivatives of PLPC were identified based on MS/MS data, namely product ions attributed to lyso-phosphatidylcholines, product ions formed by loss of
nH
2O and H
2O
2 from [MH]
+ ions groups, and product ions involving the hydroxy groups, providing information about the position of these groups and of the double bonds along the carbon chain of lineloyl moiety.