Hydrogen peroxide biosensor based on microperoxidase-11 entrapped in lipid membrane

• Published in: Biosensors & bioelectronics Vol. 18; no. 10; pp. 1225 - 1230
• Main Authors: Huang, Weimin, Jia, Jianbo, Zhang, Zheling, Han, Xiaojun, Tang, Jilin, Wang, Jianguo, Dong, Shaojun, Wang, Erkang
• Format: Journal Article
• Language: English
• Published: Lausanne Elsevier B.V 01-09-2003; Elsevier Science
• Subjects: Biological and medical sciences; Biosensing Techniques; Biosensor; Biosensors; Biotechnology; Didodecyldimethylammonium bromide; Electrodes; Fundamental and applied biological sciences. Psychology; Hydrogen Peroxide - analysis; Hydrogen-Ion Concentration; Lipid membrane; Liposomes - chemistry; Liposomes - metabolism; Membranes, Artificial; Methods. Procedures. Technologies; Microperoxidase-11; Peroxidases - chemistry; Peroxidases - metabolism; Quaternary Ammonium Compounds - chemistry; Quaternary Ammonium Compounds - metabolism; Spectroscopy, Fourier Transform Infrared; Time Factors; Various methods and equipments; Microperoxidase-11; Lipid membrane; Biosensor; Didodecyldimethylammonium bromide; Hydrogen peroxide; Peroxidases; Enzyme; Immobilization; Lipids; Membrane; Oxidoreductases; Didodecyldimeihylammonium bromide; Enzyme electrode
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/S0956-5663(03)00070-8

A highly catalytic activity microperoxidase-11 (MP-11) biosensor for H
2O
2 was developed to immobilizing the heme peptide in didodecyldimethylammonium bromide (DDAB) lipid membrane. The enzyme electrode thus obtained responded to H
2O
2 without electron mediator or promoter, at a potential of +0.10 V versus Ag
∣
AgCl. A linear calibration curve is obtained over the range from 2.0×10
−5 to 2.4×10
−3 M. The biosensor responds to hydrogen peroxide in 15 s and has a detection limit of 8×10
−7 M (S/N=3) Providing a natural environment with lipid membrane for protein immobilization and maintenance of protein functions is a suitable option for the design of biosensors.