HPLC determination of a novel aroylhydrazone iron chelator ( o-108) in rabbit plasma and its application to a pilot pharmacokinetic study

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Vol. 838; no. 2; pp. 107 - 112
• Main Authors: KOVARIKOVA, Petra, KLIMES, Jiri, STERBA, Martin, POPELOVA, Olga, GERSL, Vladimir, PONKA, Premysl
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 11-07-2006; Elsevier Science
• Subjects: Analysis; Analytical, structural and metabolic biochemistry; Animals; Area Under Curve; Biological and medical sciences; Chromatography, High Pressure Liquid - methods; Drug Stability; Fundamental and applied biological sciences. Psychology; General pharmacology; HPLC; Hydrazones - blood; Hydrazones - chemistry; Hydrazones - pharmacokinetics; Iron Chelating Agents - analysis; Iron Chelating Agents - chemistry; Iron Chelating Agents - pharmacokinetics; Iron chelator; Male; Medical sciences; Molecular Structure; o-108; Pharmacokinetics; Pharmacology. Drug treatments; Pilot Projects; Pyridoxal - analogs & derivatives; Pyridoxal - blood; Pyridoxal - pharmacokinetics; Pyridoxal 2-chlorobenzoyl hydrazone; Rabbits; Reproducibility of Results; Pyridoxal 2-chlorobenzoyl hydrazone; o-108; Pharmacokinetics; Iron chelator; HPLC; Biological fluid; HPLC chromatography; Rabbit; Iron; Lagomorpha; Blood plasma; Vertebrata; Mammalia; Animal; Chelating agent; Quantitative analysis
• ISSN: 1570-0232; 1873-376X
• DOI: 10.1016/j.jchromb.2006.04.020

A high performance liquid chromatographic method for the determination of a biocompatible iron chelator, pyridoxal 2-chlorobenzoyl hydrazone (
o-108), in rabbit plasma was developed and validated. The separation was achieved on a C18 column with the mobile phase composed of a mixture of 0.01
M phosphate buffer (pH 6) with the addition of EDTA (2
mM), methanol and acetonitrile (42:24:14; v/v/v). The method was validated with respect to selectivity, linearity (0.8–150
μg/mL), intra- and inter-day variability and stability. This method was successfully applied to the analysis of the samples obtained from a pilot pharmacokinetic experiment, in which the chelator was administered intravenously to rabbits.