Multiple target-site mutations occurring in lepidopterans confer resistance to diamide insecticides

• Published in: Insect biochemistry and molecular biology Vol. 121; p. 103367
• Main Authors: Huang (黄镜梅), Jing-Mei, Rao (饶聪), Cong, Wang (王帅), Shuai, He (何林凤), Lin-Feng, Zhao (赵思琪), Si-Qi, Zhou (周丽琪), Li-Qi, Zhao (赵云霞), Yun-Xia, Yang (杨凤霞), Feng-Xia, Gao (高聪芬), Cong-Fen, Wu (吴顺凡), Shun-Fan
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01-06-2020
• Subjects: Amino Acid Sequence; Animals; Benzamides - pharmacology; Chilo suppressalis; Chlorantraniliprole; CRISPR-Cas Systems; CRISPR/Cas9; Diamide resistance; Drosophila melanogaster - genetics; Drosophila melanogaster - metabolism; Insect Proteins - chemistry; Insect Proteins - genetics; Insect Proteins - metabolism; Insecticide Resistance - genetics; Insecticides - pharmacology; Moths - drug effects; Moths - genetics; Moths - metabolism; Mutation; ortho-Aminobenzoates - pharmacology; Pyrazoles - pharmacology; Ryanodine receptor; Ryanodine Receptor Calcium Release Channel - chemistry; Ryanodine Receptor Calcium Release Channel - genetics; Ryanodine Receptor Calcium Release Channel - metabolism; Sequence Alignment; Sulfones - pharmacology; Target-site mutation; Chlorantraniliprole; Target-site mutation; Chilo suppressalis; CRISPR/Cas9; Diamide resistance; Ryanodine receptor
• ISSN: 0965-1748; 1879-0240
• DOI: 10.1016/j.ibmb.2020.103367

Diamide resistant phenotypes have evolved in the field and the resistance has been attributed to target-site mutations in some lepidopteran pests. In this study, we documented the resistance status of Chilo suppressalis to chlorantraniliprole during 2016–2018 in seven provinces of China. To investigate the possible role of target-site mutations as known from lepidopterans, we sequenced respective domains of the RyR gene of C. suppressalis with different levels of diamide resistance. The results revealed that I4758M (corresponding to I4790M in P. xylostella), Y4667D/C (numbered according to C. suppressalis), G4915E (corresponding to G4946E in P. xylostella), and one novel Y4891F (numbered according to C. suppressalis) RyR target-site mutations were present. The contribution of these mutations was further investigated by diamide toxicity bioassays with eight genome modified Drosophila melanogaster lines. The study showed that genome modified flies bearing the Y4667D mutation (corresponding to the Y4667D and I4758M simultaneous mutation in C. suppressalis) exhibited high resistance ratios to chlorantraniliprole (1542.8-fold), cyantraniliprole (487.9-fold) and tetrachlorantraniliprole (290.1-fold). The M4758I and G4915E simultaneous mutations (corresponding to single G4915E mutation in C. suppressalis) showed high resistance ratios to chlorantraniliprole (153.1-fold) and cyantraniliprole (323.5-fold), and relatively low resistance to flubendiamide (28.9-fold) and tetrachlorantraniliprole (25.2-fold). These findings suggest that multiple point mutations in RyR confer diamide resistance of C. suppressalis. The results contribute to a better understanding of insect diamide resistance mechanisms and provide insights on the impact of RyR target-site mutations in insects.
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•Most of C. suppressalis field populations had developed high level of resistance to chlorantraniliprole.•Five mutations, I4758M, Y4667D, Y4667C, G4915E, and Y4891F are present in C. suppressalis populations.•Y4667D mutation confers very high resistance to chlorantraniliprole in Drosophila.•Double mutation, M4758I and G4915E, confers high chlorantraniliprole resistance in Drosophila.