Growth-inhibitory effects of MOB2 on human hepatic carcinoma cell line SMMC-7721

• Published in: World journal of gastroenterology : WJG Vol. 18; no. 48; pp. 7285 - 7289
• Main Authors: Leng, Jian-Jun, Tan, Hua-Min, Chen, Ke, Shen, Wei-Gan, Tan, Jing-Wang
• Format: Journal Article
• Language: English
• Published: United States Baishideng Publishing Group Co., Limited 28-12-2012
• Subjects: Apoptosis; Brief; Carcinoma, Hepatocellular - genetics; Carcinoma, Hepatocellular - metabolism; Cell Line, Tumor; Cell Proliferation; Cloning, Molecular; DNA, Complementary - metabolism; Flow Cytometry; Gene Expression Regulation, Neoplastic; Gene Transfer Techniques; Genetic Therapy; Genetic Vectors; Human Umbilical Vein Endothelial Cells; Humans; Liver Neoplasms - genetics; Liver Neoplasms - metabolism; Nerve Tissue Proteins - genetics; Nerve Tissue Proteins - metabolism; SMMC-7721细胞; Transfection; 人肝癌细胞株; 人脐静脉内皮细胞; 基因治疗; 抑制效果; 生长抑制; 脂质体转染; 诱导凋亡; Gene expression; Growth inhibition; SMMC-7721; Apoptosis
• ISSN: 1007-9327; 2219-2840
• DOI: 10.3748/wjg.v18.i48.7285

AIM：To investigate the growth-inhibiting and apoptosis-inducing effects of the gene MOB2 on human hepatic carcinoma cell line SMMC-7721.METHODS：The full-length cDNA of the MOB2 gene was amplified from human umbilical vein endothelial cells.The correct full-length MOB2 cDNA was subcloned into the eukaryotic expression vector pEGFP-C1.After lipofection of the MOB2 gene into cancer cells,the levels of MOB2 protein in the cancer cells were detected by immunoblotting.To transfect the recombined plasmid vector pEGFP-CI-MOB2 into SMMC-7721 cells,the cells were cultured in Dulbecco＇s Modified Eagle＇sMedium with 10% fetal calf serum and glutamine,and then mixed with liposomes,Lipofectamine 2000 and the plasmid vector pEGFP-CI-MOB2.RESULTS：We observed the growth and proliferation of SMMC-7721 cells containing pEGFP-CI-MOB2 and analyzed their apoptosis and growth cycle phases by flow cytometry.We successfully transfected the recombined plasmid vector pEGFP-CI-MOB2 into SMMC-7721 cells and screened for a single clone cell containing MOB2.After transfection,MOB2 enhanced growth suppression,induced apoptosis,increased the ratio of G0/G1,significantly inhibited the advance of cell cycle phase,and arrested cells in G0/G1 phase.CONCLUSION：MOB2 overexpression induces apoptosis and inhibits the growth of human hepatic cancer cells,which may be useful in gene therapy for hepatic carcinoma.