An ancestral role in peroxisome assembly is retained by the divisional peroxin Pex11 in the yeast Yarrowia lipolytica

• Published in: Journal of cell science Vol. 128; no. 7; pp. 1327 - 1340
• Main Authors: Chang, Jinlan, Klute, Mary J, Tower, Robert J, Mast, Fred D, Dacks, Joel B, Rachubinski, Richard A
• Format: Journal Article
• Language: English
• Published: England 01-04-2015
• Subjects: Evolution, Molecular; Fungal Proteins - genetics; Fungal Proteins - metabolism; Membrane Proteins - genetics; Membrane Proteins - metabolism; Molecular Sequence Data; Peroxisomes - genetics; Peroxisomes - metabolism; Phylogeny; Protein Transport; Yarrowia - genetics; Yarrowia - metabolism; Yarrowia lipolytica; Comparative genomics; Peroxisome; Organelle biogenesis; Pex11; Yarrowia lipolytica
• ISSN: 0021-9533; 1477-9137
• DOI: 10.1242/jcs.157743

The peroxin Pex11 has a recognized role in peroxisome division. Pex11p remodels and elongates peroxisomal membranes prior to the recruitment of dynamin-related GTPases that act in membrane scission to divide peroxisomes. We performed a comprehensive comparative genomics survey to understand the significance of the evolution of the Pex11 protein family in yeast and other eukaryotes. Pex11p is highly conserved and ancestral, and has undergone numerous lineage-specific duplications, whereas other Pex11 protein family members are fungal-specific innovations. Functional characterization of the in-silico-predicted Pex11 protein family members of the yeast Yarrowia lipolytica, i.e. Pex11p, Pex11Cp and Pex11/25p, demonstrated that Pex11Cp and Pex11/25p have a role in the regulation of peroxisome size and number characteristic of Pex11 protein family members. Unexpectedly, deletion of PEX11 in Y. lipolytica produces cells that lack morphologically identifiable peroxisomes, mislocalize peroxisomal matrix proteins and preferentially degrade peroxisomal membrane proteins, i.e. they exhibit the classical pex mutant phenotype, which has not been observed previously in cells deleted for the PEX11 gene. Our results are consistent with an unprecedented role for Pex11p in de novo peroxisome assembly.