Aptamer-molecularly imprinted sensor base on electrogenerated chemiluminescence energy transfer for detection of lincomycin

• Published in: Biosensors & bioelectronics Vol. 91; pp. 687 - 691
• Main Authors: Li, Shuhuai, Liu, Chunhua, Yin, Guihao, Zhang, Qun, Luo, Jinhui, Wu, Nanchun
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 15-05-2017
• Subjects: Aminophenols - chemistry; Animals; Anti-Bacterial Agents - analysis; Aptamer; Aptamers, Nucleotide - chemistry; Biosensing Techniques - methods; Dual recognition; Electrochemical Techniques - methods; Electrodes; Energy Transfer; Food Contamination - analysis; Gold - chemistry; Graphite - chemistry; Limit of Detection; lincomycin; Lincomycin - analysis; Luminescence; Luminescent Measurements - methods; Meat - analysis; Molecular Imprinting - methods; Molecularly imprinted; Nanostructures - chemistry; Polymerization; Dual recognition; Aptamer; Energy transfer; Molecularly imprinted; lincomycin
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/j.bios.2017.01.038

In this study, a biosensor with a dual recognition system comprising a molecularly imprinted polymer (MIP) and aptamers selective for lincomycin was fabricated. The MIP was synthesized by electropolymerization of carbon dots (C-dots)-tagged DNA aptamers combined with lincomycin and o-aminophenol on the gold-nanoparticle-functionalized graphene oxide (Au-GO)-modified electrode. Electrogenerated chemiluminescence (ECL) resonance energy transfer was observed between Au-GO and C-dots. After the C-dots accepted the energy, they acted as a signal indicator and exhibited enhanced signal intensity in the presence of target lincomycin. When lincomycin was competitively bound to DNA aptamers and MIP, it blocked the transfer of energy, and a decreased ECL signal was observed. Hence, a dual recognition method for the detection of lincomycin is realized. Using this strategy, the sensor exhibited a linear ECL response to lincomycin at concentrations from 5.0 × 10 −12 mol/L to 1.0 × 10 −9 mol/L. The detection limit of this assay was found to be 1.6 × 10 −13 mol/L. This method was utilized to determine lincomycin residuals in meat samples with satisfactory results.
•A new aptamer-molecularly imprinted sensor for lincomycin detection was first reported.•The aptasensor presents a generic detection strategy for detecting pesticide residues.•A dual recognition system was obtained from the DNA aptamers and MIPs for the target molecule.