Lipopolysaccharide-induced impairment of classical swine fever virus infection in monocytic cells is sensitive to 2-aminopurine

• Published in: Antiviral research Vol. 53; no. 1; pp. 75 - 81
• Main Authors: Knoetig, Sonja M, McCullough, Kenneth C, Summerfield, Artur
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 2002; Elsevier
• Subjects: 2-aminopurine; 2-Aminopurine - pharmacology; Animals; Antibiotics. Antiinfectious agents. Antiparasitic agents; Antiviral agents; Antiviral effect; Biological and medical sciences; Cells, Cultured; Classical Swine Fever - virology; Classical swine fever virus; Classical swine fever virus - drug effects; Classical swine fever virus - pathogenicity; Classical swine fever virus - physiology; Cytokines - biosynthesis; IFN; Interferons - biosynthesis; Lipopolysaccharides - immunology; LPS; Macrophages - immunology; Macrophages - virology; Medical sciences; Monocytes - immunology; Monocytes - virology; Monocytes/macrophages; Pharmacology. Drug treatments; PKR (double-stranded RNA-dependent protein kinase); Poly(l)·poly(C); Virus Replication - drug effects; IFN; PKR (double-stranded RNA-dependent protein kinase); Classical swine fever virus; Antiviral effect; Monocytes/macrophages; LPS; 2-aminopurine; Poly(l)·poly(C); Pestivirus; Monocyte; Enzyme; Transferases; Cytokine; Adenine; Enzyme inhibitor; Activation; Infection; Virus; Protein kinase; Viral disease; Lipopolysaccharide; Antiviral; Replication; Interferon; Flaviviridae; Macrophage; Hog cholera virus
• ISSN: 0166-3542; 1872-9096
• DOI: 10.1016/S0166-3542(01)00193-0

Lipopolysaccharide (LPS) impairs classical swine fever virus (CSFV) replication in monocytic cells, which are primary targets for CSFV and mediators of virus-induced immunomodulation. Although soluble antiviral factors including interferons (IFN) were not detected, IFN-α and IFN-β mRNA were induced. The serine threonine protein kinase inhibitor 2-aminopurine, impeded this antiviral activity. These results indicate that the LPS-induced antiviral state employs signaling pathways, in which the double-stranded RNA-dependent protein kinase (PKR) is actively involved.