l-Cysteine capped Mn-doped ZnS quantum dots as a room temperature phosphorescence sensor for in-vitro binding assay of idarubicin and DNA

• Published in: Biosensors & bioelectronics Vol. 70; pp. 345 - 350
• Main Authors: Ertas, Nusret, Satana Kara, Hayriye Eda
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 15-08-2015
• Subjects: Cysteine - chemistry; DNA; DNA - analysis; DNA - chemistry; DNA binding; Drug Interactions; Drugs; Dynamics; Idarubicin; Idarubicin - analysis; Idarubicin - chemistry; l-cysteine capped Mn doped ZnS quantum dots; Luminescent Measurements - methods; Manganese; Manganese - chemistry; Nanostructure; Phosphorescence; Quantum Dots; Quenching; Room temperature phosphorescence sensor; Selenium Compounds - chemistry; Zinc Compounds - chemistry; Zinc sulfides; l-cysteine capped Mn doped ZnS quantum dots; Idarubicin; Room temperature phosphorescence sensor; DNA; DNA binding
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/j.bios.2015.03.055

l-cysteine capped Mn doped ZnS quantum dots/ Idarubicin (IDA) nanohybrids were used as novel room temperature phosphorescence (RTP) sensor to detect double stranded deoxyribonucleic acid (ds-DNA)/drug interaction. IDA, anthracycline derivative anticancer drug, was adsorbed on the surface of the QDs as an electron acceptor to quench the RTP emission. The RTP intensity of QDs was quenched quickly upon addition of quencher and the reaction reached equilibrium within 2min. The quenching mechanism of phosphorescence of Mn-doped ZnS QDs by IDA is a combined dynamic and static quenching. The static and dynamic quenching constants were found as 1.1×105M−1 and 8.7×104M−1, respectively. The addition of ds-DNA caused formation of ds-DNA/IDA complex and recovered the RTP signal of Mn-doped ZnS QDs, which allowed qualitative analysis. Under optimal conditions, RTP intensity of QDs/IDA nanohybrids increased linearly with the concentration of ds-DNA from 1.2 to 6.0µM. This method is simple, low cost and avoids from interferences.
•l-cysteine capped Mn doped ZnS quantum dots/ Idarubicin nanohybrids was used for DNA binding evaluation.•This sensor is simple, low cost and not affected by interferences such as autofluorescence and light scattering.•The interaction between idarubicin–quantum dots and idarubicin–DNA are evaluated.•The interaction of idarubicin and DNA is intercalation mode.