Transfer of condensed viral DNA into eukaryotic cells using proteoliposomes

• Published in: Gene Vol. 63; no. 2; p. 321
• Main Authors: Tikchonenko, T I, Glushakova, S E, Kislina, O S, Grodnitskaya, N A, Manykin, A A, Naroditsky, B S
• Format: Journal Article
• Language: English
• Published: Netherlands 31-03-1988
• Subjects: Adenoviruses, Simian - genetics; DNA, Viral - drug effects; DNA, Viral - genetics; Genes, Viral; Hemagglutinin Glycoproteins, Influenza Virus; Hemagglutinins, Viral - genetics; Influenza A virus - genetics; Liposomes; Proteolipids; Simian virus 40 - genetics; Spermine - pharmacology; Transfection
• ISSN: 0378-1119
• DOI: 10.1016/0378-1119(88)90535-5

High-molecular-weight viral DNAs have been packed into proteoliposomes prepared by reverse-phase evaporation followed by phospholipid membrane targeting by influenza virus glycoprotein bound to hydrophobic 'anchors'. DNA has been encapsulated in the form of spermine condensates--toroidal structures sized approx. 0.1 micron, resistant to ultrasound. The efficiency of entrapping into liposomes reached 30% for condensed DNA of Mr up to 3 X 10(7). Specific infectivity of simian virus 40 DNA and simian adenovirus DNA packed into such proteoliposomes was 50- to 100-fold higher than that shown by free DNA preparations under Ca.phosphate-precipitation conditions.