Bovine papillomavirus E5 oncoprotein binds to the activated form of the platelet-derived growth factor β receptor in naturally occurring bovine urinary bladder tumours

• Published in: Oncogene Vol. 25; no. 8; pp. 1251 - 1260
• Main Authors: BORZACCHIELLO, G, RUSSO, V, GENTILE, F, ROPERTO, F, VENUTI, A, NITSCH, L, CAMPO, M. S, ROPERTO, S
• Format: Journal Article
• Language: English
• Published: Basingstoke Nature Publishing 23-02-2006; Nature Publishing Group
• Subjects: Adenocarcinoma - metabolism; Adenocarcinoma - veterinary; Adenocarcinoma - virology; Animal models; Animals; Biological and medical sciences; Bladder; Bladder cancer; Bovine papillomavirus; Carcinogenesis; Carcinoma, Papillary - metabolism; Carcinoma, Papillary - veterinary; Carcinoma, Papillary - virology; Cattle; Cell physiology; Cell receptors; Cell structures and functions; Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes; Confocal microscopy; Disease Models, Animal; DNA, Viral - genetics; DNA, Viral - metabolism; Fundamental and applied biological sciences. Psychology; Growth factors; Hemangiosarcoma - metabolism; Hemangiosarcoma - veterinary; Hemangiosarcoma - virology; Immunization; Immunoprecipitation; Medical sciences; Miscellaneous; Molecular and cellular biology; Nephrology. Urinary tract diseases; Oncogene Proteins, Viral - genetics; Oncogene Proteins, Viral - metabolism; Oncoproteins; Phosphorylation; Platelet-derived growth factor; Receptor, Platelet-Derived Growth Factor beta - metabolism; Tumors; Tumors of the urinary system; Urinary bladder; Urinary Bladder - metabolism; Urinary Bladder Neoplasms - metabolism; Urinary Bladder Neoplasms - veterinary; Urinary Bladder Neoplasms - virology; Urinary tract. Prostate gland; Vaccination; Papovaviridae; Carcinogenesis; Papillomavirus; E5; Urinary bladder; Bovine papillomavirus 2; urinary bladder tumours; Ungulata; Platelet derived growth factor; Biological receptor; Urinary system disease; Bovine; Urinary tract disease; PDGF β receptor; Bladder tumor; Virus; Vertebrata; Bovine papillomavirus; Mammalia; bovine papillomavirus type 2; Urinary system; C-Onc gene; Bladder disease; Artiodactyla; Onc gene
• ISSN: 0950-9232; 1476-5594
• DOI: 10.1038/sj.onc.1209152

Studies regarding the functions of the bovine papillomavirus (BPV) E5 oncoprotein in vivo are lacking and no E5-mediated mechanism underlying epithelial carcinogenesis is known. We have shown that BPV-2 DNA is present in the majority of naturally occurring urinary bladder tumours of cattle and that E5 is expressed in the cancer cells. Here we show that the interaction between the platelet-derived growth factor (PDGF) beta receptor and BPV E5, described in vitro in cultured cells, takes place in vivo in bovine urinary bladder cancers. In these cancers, E5 and PDGF beta receptor colocalize, as shown by confocal microscopy, and physically interact, as shown by coimmunoprecipitation. Furthermore, the PDGF beta receptor associated with E5 is highly phosphorylated, suggesting the functional activation of the receptor upon E5 interaction. Our results demonstrate, for the first time, that E5-PDGF beta receptor interaction occurs during the natural history of bovine urinary bladder tumours, suggesting an important role for E5 in carcinogenesis. Finally, the system provides a suitable animal model of papillomavirus-associated cancer to test therapeutic vaccination against E5. Successful bladder tumour regression would provide a valuable model for therapeutic vaccination against papillomavirus-associated tumours.