Simultaneous determination of selected veterinary antibiotics in gilthead seabream ( Sparus Aurata) by liquid chromatography–mass spectrometry

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Vol. 857; no. 1; pp. 142 - 148
• Main Authors: ROMEROGONZALEZ, R, LOPEZMARTINEZ, J, GOMEZMILAN, E, GARRIDOFRENICH, A, MARTINEZVIDAL, J
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 15-09-2007; Elsevier Science
• Subjects: Analysis; Analytical, structural and metabolic biochemistry; Animals; Anti-Bacterial Agents - analysis; Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - therapeutic use; Antibiotics; Biological and medical sciences; Chromatography, High Pressure Liquid - methods; Drug Residues - analysis; Drug Residues - chemistry; Fish Diseases - drug therapy; Fish Diseases - metabolism; Fishes - metabolism; Fundamental and applied biological sciences. Psychology; General pharmacology; Gilthead sea bream; Liquid chromatography; Mass spectrometry; Medical sciences; Molecular Structure; Multiclass; Muscles - chemistry; Oxytetracycline - analysis; Pharmacology. Drug treatments; Quinolones - analysis; Reference Standards; Reproducibility of Results; Sea Bream - metabolism; Sensitivity and Specificity; Solid Phase Extraction; Spectrometry, Mass, Electrospray Ionization - methods; Sulfonamides - analysis; Tandem Mass Spectrometry - methods; Tetracyclines - analysis; Trimethoprim - analysis; Veterinary Drugs - analysis; Veterinary Drugs - chemistry; Veterinary Drugs - therapeutic use; Liquid chromatography; Antibiotics; Multiclass; Mass spectrometry; Gilthead sea bream; Edible fish; Chemical analysis; Sea bream; Veterinary medicine; Vertebrata; Antibiotic; Sparus aurata; Pisces; Simultaneous measurement
• ISSN: 1570-0232; 1873-376X
• DOI: 10.1016/j.jchromb.2007.07.011

A method was optimised and validated for simultaneous monitoring of several drugs of different classes of antibiotics such as quinolones (oxilinic acid and flumequine), tetracyclines (oxytetracycline), sulfonamides (sulfadiazine) and trimethoprim in fish muscle and skin. The method is based on solid–liquid extraction without further sample clean up followed by liquid chromatography–mass spectrometry (LC–MS) determination with electrospray ion source (ESI) in positive mode. The limits of quantification (LOQs) were lower than 20
μg/kg for all compounds and repeatability, expressed as relative standard deviations (RSD), were lower than 15%. Therefore, the LC–MS method was successfully applied for the quantitative determination of antibiotics in gilthead sea bream muscle and skin and oxytetracycline in medicated fishes.