Diversity of Sinorhizobium meliloti and S. medicae nodulating Medicago truncatula according to host and soil origins

• Published in: World journal of microbiology & biotechnology Vol. 21; no. 6-7; pp. 1009 - 1015
• Main Authors: Zribi, K, Mhamdi, R, Huguet, T, Aouani, M.E
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer 01-10-2005; Springer Nature B.V
• Subjects: Alfalfa; Bacteria; Biological and medical sciences; Biotechnology; Bulla; Clustering; Fundamental and applied biological sciences. Psychology; Genetic diversity; genetic variation; Genetics; Genotypes; Medicago truncatula; Microbiology; Natural populations; nodulation; Origins; Plant populations; Plants (organisms); Polymerase chain reaction; Populations; restriction fragment length polymorphism; Sinorhizobium medicae; Sinorhizobium meliloti; Soil (material); soil bacteria; Soils; Species diversity; Tunisia; Medicago truncatula; Sinorhizobium medicae; Genetic diversity; soil; Polymerase chain reaction; Soils; Leguminosae; Dicotyledones; plant host population; Angiospermae; repetitive extragenic palindromic- polymerase chain reaction (REP-PCR); Bacteria; Spermatophyta; Rhizobiaceae; Sinorhizobium meliloti
• ISSN: 0959-3993; 1573-0972
• DOI: 10.1007/s11274-004-7653-4

The model legume Medicago truncatula was used to trap natural populations of Sinorhizobium meliloti and Sinorhizobium medicae in Tunisian soils to explore their genetic diversity. About 155 Sinorhizobium isolates were trapped from a combination of three soils and four Medicago truncatula populations in order to analyse soil and plant population effects on nodulating Sinorhizobium diversity. The species assignment was done according to the restriction fragment length polymorphism analysis of polymerase chain reaction (PCR/RFLP) of 16S rRNA genes and their infraspecific genetic diversity was assessed with the repetitive extragenic palindromic-polymerase chain reaction (REP-PCR) technique. It appeared that the trapped bacteria were clustered according to the soil of origin, particularly Sinorhizobium medicae isolates. However, regarding the plant population effect, it appeared that no major clustering tendency could be suggested even if the Bulla Regia and Soliman Medicago truncatula populations appeared to nodulate together specific Sinorhizobium medicae genotypes.