Simultaneous determination of zidovudine and lamivudine in human serum using HPLC with tandem mass spectrometry

• Published in: Journal of pharmaceutical and biomedical analysis Vol. 22; no. 6; pp. 967 - 983
• Main Authors: Kenney, Kathryn B, Wring, Stephen A, Carr, Richard M, Wells, Guy N, Dunn, John A
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01-07-2000; Elsevier Science
• Subjects: AIDS/HIV; Analysis; Anti-HIV Agents - blood; Antibiotics. Antiinfectious agents. Antiparasitic agents; Antibody Specificity; Antiviral agents; Biological and medical sciences; Calibration; Chromatography, High Pressure Liquid; Determination; General pharmacology; HIV; HPLC; Human immunodeficiency virus; Human plasma; Humans; Indicators and Reagents; Isotope Labeling; Lamivudine; Lamivudine - blood; LC-MS/MS; Mass Spectrometry; Medical sciences; Pharmacology. Drug treatments; Radioimmunoassay; Reference Standards; Reproducibility of Results; Spectrophotometry, Ultraviolet; Zidovudine; Zidovudine - blood; LC-MS/MS; Human plasma; HIV; Lamivudine; Determination; Zidovudine; HPLC; Human; Validation; Spraying; Oral administration; HPLC chromatography; Electrospray; Blood plasma; Electric field; Ultraviolet detector; Ionization; Dideoxynucleoside; Radioimmunoassay; Mass spectrometry MS/MS; Dosage form; Antiviral; Tablet; Pharmacokinetics; Pyrimidine nucleoside; Quantitative analysis
• ISSN: 0731-7085; 1873-264X
• DOI: 10.1016/S0731-7085(00)00248-X

A method employing high performance liquid chromatography (HPLC) with tandem mass spectrometry (MS) has been developed and validated for the simultaneous determination of clinically relevant levels of zidovudine (AZT) and lamivudine (3TC) in human serum. The method incorporates a fully automated ultrafiltration sample preparation step that replaces the solid-phase extraction step typically used for HPLC with UV detection. The calibration range of the dual-analyte LC-MS/MS method is 2.5–2500 and 2.5–5000 ng ml
−1 for AZT and 3TC, respectively, using 0.25 ml of human serum. The lower limit of quantification was 2.5 ng ml
−1 for each analyte, with a chromatographic run time of approximately 6 min. Overall accuracy, expressed as bias, and inter- and intra-assay precision are <±7 and <10% for AZT, and <±5 and <12.1% for 3TC over the full concentration ranges. A cross-validation study demonstrated that the LC-MS/MS method afforded equivalent results to established methods consisting of a radioimmuno-assay for AZT and an HPLC-UV method for 3TC. Moreover, the LC-MS/MS was more sensitive, allowed markedly higher-throughput, and required smaller sample volumes (for 3TC only). The validated method has been used to support post-marketing clinical studies for Combivir™ — a combination tablet containing AZT and 3TC.