Agar/κ-carrageenan/montmorillonite nanocomposite hydrogels for wound dressing applications

• Published in: International journal of biological macromolecules Vol. 164; pp. 4591 - 4602
• Main Authors: Polat, Tülin Gürkan, Duman, Osman, Tunç, Sibel
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01-12-2020
• Subjects: Agar - administration & dosage; Agar - pharmacology; Agar - toxicity; Anti-Bacterial Agents - pharmacology; Antibacterial; Bandages, Hydrocolloid; Bentonite - administration & dosage; Bentonite - pharmacology; Carrageenan - administration & dosage; Carrageenan - pharmacology; Carrageenan - toxicity; Cell Line, Tumor; Chloramphenicol - administration & dosage; Chloramphenicol - pharmacokinetics; Drug Liberation; Drug release; Drug Storage; Escherichia coli - drug effects; Humans; Hydrogels - chemistry; Hydrogels - pharmacology; Hydrogels - toxicity; Lidocaine - administration & dosage; Lidocaine - pharmacokinetics; Materials Testing; Microscopy, Electron, Scanning; Montmorillonite; Nanocomposite hydrogel; Nanocomposites - toxicity; Nanogels - toxicity; Osteoblasts - drug effects; Polysaccharide; Solutions; Staphylococcus aureus - drug effects; Stress, Mechanical; Temperature; Wound dressing material; Wound Infection - prevention & control; Drug release; Nanocomposite hydrogel; Antibacterial; Montmorillonite; Polysaccharide; Wound dressing material
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2020.09.048

In this study, agar/κ-carrageenan/montmorillonite (MMT) hydrogels were prepared to examine their usability as wound dressing materials and to see the effect of MMT amount on some properties of agar/κ-carrageenan hydrogel materials. Hydrogels were characterized by SEM-EDX, TEM and DSC analyses. By increasing the MMT content within hydrogel matrix from 0% to 5%, the decomposition temperature of the hydrogel material was increased from 256.6 °C to 262.1 °C. Swelling amount of hydrogels in d-glucose solution (2682%) was found to be much higher compared with other physiological solutions such as physiological saline solution (937%), synthetic urine solution (746%) and simulated wound fluid (563%). The release studies of analgesic lidocaine hydrochloride (LDC) and antibiotic chloramphenicol (CLP) drugs from hydrogel systems demonstrated that the release amount of LDC and CLP from hydrogels could be controlled by MMT amount within hydrogel matrix. The concentrations of drugs within hydrogel sample stored at 4 °C for 6 months did not exhibit a significant change. Hydrogel materials containing CLP exhibited good antibacterial activity against E. coli and S. aureus. Cytotoxicity test results indicated that hydrogels were biocompatible with MG-63 cells. The ultimate compressive stress of agar/κ-carrageenan hydrogel with LDC and CLP and agar/κ-carrageenan/MMT hydrogel including 5% MMT with LDC and CLP was measured as 38.30 kPa and 47.70 kPa, respectively. The experimental results revealed that prepared agar/κ-carrageenan and agar/κ-carrageenan/MMT hydrogels have great potential for wound care applications.
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•Agar/κ-carrageenan/MMT nanocomposite hydrogels as wound dressing materials were developed.•Swelling amount of agar/κ-carrageenan hydrogel in simulated wound fluid was 563%.•Hydrogels with CLP showed good antibacterial activity against E. coli and S. aureus.•The release of drugs from hydrogels was reduced by the addition of MMT.•Hydrogels did not show cytotoxic behavior against MG-63 cells.