Effects of juvenile hormone and 20-hydroxyecdysone on alkaline phosphatase activity in Drosophila under normal and heat stress conditions

• Published in: Journal of insect physiology Vol. 53; no. 6; pp. 587 - 591
• Main Authors: Rauschenbach, I.Yu, Bogomolova, E.V., Gruntenko, N.E., Adonyeva, N.V., Chentsova, N.A.
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01-06-2007
• Subjects: 20-Hydroxyecdysone; Alkaline phosphatase; Alkaline Phosphatase - metabolism; Animals; Dopamine; Dopamine - metabolism; Drosophila; Drosophila melanogaster; Drosophila melanogaster - drug effects; Drosophila melanogaster - enzymology; Drosophila melanogaster - metabolism; Drosophila virilis; ecdysterone; Ecdysterone - pharmacology; enzyme activity; Female; Heat stress; heat stress response; hormonal regulation; Hot Temperature; Juvenile hormone; juvenile hormones; Juvenile Hormones - pharmacology; mutants; Alkaline phosphatase; 20-Hydroxyecdysone; Dopamine; Heat stress; Drosophila; Juvenile hormone
• ISSN: 0022-1910; 1879-1611
• DOI: 10.1016/j.jinsphys.2007.02.011

The effect of 20-hydroxyecdysone (20E) and the juvenile hormone (JH) on the activity of the alkaline phosphatase (ALP) has been studied in young females of wild-type
Drosophila virilis and
Drosophila melanogaster under normal conditions and under heat stress (38
°C). Both 20E feeding of the flies and JH application led to a substantial rise in ALP activity. ALP activity was also measured in young females of a JH-deficient strain of
D. melanogaster,
apterous
56
f
. A decrease in the enzyme activity was observed in the mutant females as compared to wild type. A rise in JH and 20E levels was found not to prevent the response of ALP to heat stress, but to change its stress-reactivity. Mechanisms of regulation of dopamine (DA) level by gonadotropins in
Drosophila are discussed.